Protein Info for SM_b20616 in Sinorhizobium meliloti 1021

Annotation: thiamine biosynthesis oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF01266: DAO" amino acids 2 to 313 (312 residues), 166.1 bits, see alignment E=2.6e-52 PF00890: FAD_binding_2" amino acids 3 to 52 (50 residues), 31.2 bits, see alignment 2.2e-11 TIGR02352: glycine oxidase ThiO" amino acids 3 to 315 (313 residues), 258.8 bits, see alignment E=3.9e-81 PF13450: NAD_binding_8" amino acids 7 to 40 (34 residues), 24.5 bits, see alignment 3.9e-09

Best Hits

KEGG orthology group: K03153, glycine oxidase [EC: 1.4.3.19] (inferred from 100% identity to sme:SM_b20616)

Predicted SEED Role

"Glycine oxidase ThiO (EC 1.4.3.19)" in subsystem Thiamin biosynthesis (EC 1.4.3.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.3.19

Use Curated BLAST to search for 1.4.3.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92TI8 at UniProt or InterPro

Protein Sequence (325 amino acids)

>SM_b20616 thiamine biosynthesis oxidoreductase (Sinorhizobium meliloti 1021)
MRVLIRGAGVAGLTAAHELLARGAEVTIIENAAGFTGAASWYAGGMLAPWCERESAEETV
LTLGIGAADWWEAALPAGSVHRNGTLVVAQPRDGAELKRFASRTSGFRWLDAEEIAALEP
ALAGRFRQALFFPQEAHLDPRRALSALRQGLAARGAAFAGEDMDDRGFALTVDCTGASRI
GEFDGLRGVRGEMAYLQTDEVTLSRPVRMLHPRIPLYIVPRGGGLFMCGATMIESDDAGP
ITTRSLMELLNAAYSLHPAFAEARLVETGTGVRPAFTDNLPRAWRRENRITVNGLYRHGF
LLSPAMAGEVAALALGEENLRGDTR