Protein Info for SM_b20508 in Sinorhizobium meliloti 1021

Annotation: sugar ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 327 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00532: Peripla_BP_1" amino acids 27 to 307 (281 residues), 147 bits, see alignment E=8.4e-47 PF13407: Peripla_BP_4" amino acids 28 to 298 (271 residues), 80.3 bits, see alignment E=1.8e-26

Best Hits

Swiss-Prot: 53% identical to ARAF_AZOBR: ABC transporter L-arabinose-binding periplasmic protein (araF) from Azospirillum brasilense

KEGG orthology group: K10537, L-arabinose transport system substrate-binding protein (inferred from 100% identity to smk:Sinme_5259)

MetaCyc: 47% identical to arabinose ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-2-RXN [EC: 7.5.2.12, 7.5.2.13]

Predicted SEED Role

"L-arabinose-binding periplasmic protein precursor AraF (TC 3.A.1.2.2)" in subsystem L-Arabinose utilization (TC 3.A.1.2.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.12 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92W55 at UniProt or InterPro

Protein Sequence (327 amino acids)

>SM_b20508 sugar ABC transporter substrate-binding protein (Sinorhizobium meliloti 1021)
MRFIKAVALAGTVAIFAATSAFAADVKIGFIVKQPEEPWFQDEWKFADEAAKEKGFTLVK
IGAQDGEKVQSAIDNLGAQGAQGFIICTPDVKLGPGIVAKAAANDLKLMTVDDRLVNADG
SPIEDVPHMGISATKIGEAVGEAIVAEIKKRGWDMKEVGAIRVSYDQLPTAVDRVEGALS
VLKANGFPEANVFDAPQAKTDTEAALNAATVVLNKNAGIKKWVAVGLNDEAVLGAVRATE
TVGIPADSMIGIGIGGADSAINEFKKPSATGFFGTVIISPKRHGYETALNMYEWIANGKE
PEKLILTAGQLALRDNYEAVRKELGIE