Protein Info for SM_b20312 in Sinorhizobium meliloti 1021

Annotation: dihydroxyacetone kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 transmembrane" amino acids 618 to 637 (20 residues), see Phobius details PF04545: Sigma70_r4" amino acids 40 to 72 (33 residues), 25.2 bits, see alignment (E = 3.8e-09) PF08281: Sigma70_r4_2" amino acids 40 to 72 (33 residues), 32.2 bits, see alignment (E = 2.7e-11) PF13412: HTH_24" amino acids 43 to 75 (33 residues), 24.4 bits, see alignment (E = 6.9e-09) PF04198: Sugar-bind" amino acids 86 to 334 (249 residues), 206.8 bits, see alignment E=1.3e-64 PF02733: Dak1" amino acids 380 to 690 (311 residues), 393.8 bits, see alignment E=1.8e-121

Best Hits

KEGG orthology group: K05878, dihydroxyacetone kinase, N-terminal domain [EC: 2.7.1.-] (inferred from 100% identity to sme:SM_b20312)

Predicted SEED Role

"Putative DAK transcriptional regulator / Putative dihydroxyacetone kinase (EC 2.7.1.29), dihydroxyacetone binding subunit" in subsystem Dihydroxyacetone kinases (EC 2.7.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.1.29

Use Curated BLAST to search for 2.7.1.- or 2.7.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92WN4 at UniProt or InterPro

Protein Sequence (695 amino acids)

>SM_b20312 dihydroxyacetone kinase (Sinorhizobium meliloti 1021)
MTIKTPSARKGAANRSGTIDASDAIPLRYGDDPYVWACWLYYEDGMTQGEIAEAMGVSRA
TVNSYLADAREKGIVNISIEPARLASLTIAQELKRHFGLTDCLVVPSDDNARPLIDRLGA
AGAQALPKLLKSGDTLAVAWGRTVLSVGEHAGIASLQDMTVVQATGGTTASFAYTPELCA
SAVARAISARCVNITAPAVVKSPELLHMLLEEPLVQEQFATLARANRVLFGISSLRPNST
IHTSGFFESVSLQDYLAAGAVGVVAGRFIDERGRPVAGPLDHRTVGISLDLLRKIGTRIA
VAGGFDKVPALLAALRGGYVNVLITDAATGHGILRADGVTALDAKLWHRPKLVPTPSSYR
THVKKFLNNPNEVVEEMLDGAVKAHEIYLQPINGSHRALVARNGPRPGKVGLVIGGGTGH
EPCFLGYVGKGLADAVAVGNIFSSPPPDPIVQCAEAASGGEGVLFVYGNYAGDVMNFEMA
AEIAEEKGIKVKTVLTTDDVASSPVEDREGRRGVAGNFFIFKIAGAACDRGLSLEACEAV
TRKANLRTYTVGVALEPGSMPQTRRHNFEIGPDDMEVGMGIHGEPGVTRERIRSADEITD
SIMDRIFKEMKTAPGERVAVLVNSFGATPLMELYILFRRVQQRLAAKDILIEANWIGHYC
TSLDMAGASITILHLDQELSDLLHHPCETAFLKVN