Protein Info for SM_b20113 in Sinorhizobium meliloti 1021

Annotation: dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 transmembrane" amino acids 155 to 167 (13 residues), see Phobius details PF00389: 2-Hacid_dh" amino acids 50 to 308 (259 residues), 63.6 bits, see alignment E=1.6e-21 PF02826: 2-Hacid_dh_C" amino acids 113 to 283 (171 residues), 162.6 bits, see alignment E=6.6e-52

Best Hits

Swiss-Prot: 42% identical to HPPR_PLESU: Hydroxyphenylpyruvate reductase (HPPR) from Plectranthus scutellarioides

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_4054)

MetaCyc: 42% identical to hydroxyphenylpyruvate reductase (Coleus scutellarioides)
Hydroxyphenylpyruvate reductase. [EC: 1.1.1.237]; 1.1.1.237 [EC: 1.1.1.237]

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.237 or 1.1.1.95

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92X49 at UniProt or InterPro

Protein Sequence (310 amino acids)

>SM_b20113 dehydrogenase (Sinorhizobium meliloti 1021)
MTMLRDITILQAAELPEKTDLELKETFNTVRLPRDASAIGPFLSEYGARIRGIAVRHAHI
DAAMLDRLPALEIISSYSAGLDGIDVETAHARGVVVRNTSKILAEDVADLALALSISATR
GLMRGHDFVREGKWGESAFPLGRSLRSMKTGIVGLGHIGSAVAARLSVMGAPTAYYGPRR
KPVDLPYFDGIGALAAWADLLIVTCPASPETIGLVNAAVLASLGSEGYLVNVSRGTIVDE
QALITALAGNGIAGVALDVFEKEPFVPEALRTDPRVVLSPHMGSGTRETRQQMGDSMVAA
LVEHFESRSA