Protein Info for SM_b20020 in Sinorhizobium meliloti 1021

Annotation: pyruvate dehydrogenase E1 component, subunits alpha and beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 PF00676: E1_dh" amino acids 39 to 334 (296 residues), 104.1 bits, see alignment E=1.1e-33 PF02779: Transket_pyr" amino acids 374 to 546 (173 residues), 104.2 bits, see alignment E=9.4e-34 PF02780: Transketolase_C" amino acids 565 to 682 (118 residues), 119.9 bits, see alignment E=9.8e-39

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 100% identity to smk:Sinme_4150)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) / Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92XD9 at UniProt or InterPro

Protein Sequence (692 amino acids)

>SM_b20020 pyruvate dehydrogenase E1 component, subunits alpha and beta (Sinorhizobium meliloti 1021)
MAQAALKKDRRNAPDESIDWKKVVQLLLLSRALDEMEEQRLVPEKKILYQFSARGHDMAQ
ILLGLHLTGRHDAACGYYRSRPLLLALGVDPADALGSAMGRAGGYSDGRDIGVVFNYPNP
HGASALPMCGGVGTQYTPTAGWAQAITYFSEVLGKEEYQKDIAVVLGGDGSVASNGFWSA
LTIATTQGLPLLFYIEDNGFGISVPSSFQTPEGNIAGNLAGWKNLTVLDGDGSDPEEAAR
LTKGAVELVREGRMPVLLRLEVPRLEGHSFQDTQTYKSEELVRSEWAHDPLPRLRDYIVP
AMLSAEEWNEAARDAKAAAERARVEAESRPVADPETVTSHVFFEGRMQVMGGQHPAGYRP
PKTTETATGDGQRINMVTAIRRTLDHEMTVNQRVVLFGEDIGPKGGVHAVTLGLQEKFGT
ARVFDTSLSEEGIIGRAVGMALAGLVPVPEIQFRKYAEPAIEQLNDCGTIRWRTSNRFAA
PIVVRMAGGFLKCGDPWHSQTNEVAFVHQPGWKIAVPSNAEDAVGLLRTALRGNDPVIFF
EHRAMLDHPWARRPYPGDAFALPFGKAKFTREGRDITIVTWGAMVPRCEEAAEGISADVI
DLRTLMPWDRKAVIASVRRTRRCLIVHEDLATAGFGAEIAAAVADEAFIDLDAPISRLTM
PDIPSPHNPALLDWAVPSTERIRRKIIDLLEF