Protein Info for SM_b20005 in Sinorhizobium meliloti 1021

Annotation: glutathione S-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02798: GST_N
  • amino acids 1 to 73 (73 residues), 61.9 bits, E=1.8e-20
• PF13417: GST_N_3
  • amino acids 3 to 77 (75 residues), 47 bits, E=7.8e-16
• PF13409: GST_N_2
  • amino acids 9 to 74 (66 residues), 56.8 bits, E=8.1e-19
• PF00043: GST_C
  • amino acids 123 to 189 (67 residues), 39.3 bits, E=2e-13
• PF13410: GST_C_2
  • amino acids 128 to 184 (57 residues), 35.9 bits, E=2e-12
• PF14497: GST_C_3
  • amino acids 131 to 196 (66 residues), 27.6 bits, E=8.5e-10

Best Hits

Swiss-Prot: 68% identical to GSTA_RHILE: Protein GstA (gstA) from Rhizobium leguminosarum

KEGG orthology group: K00799, glutathione S-transferase [EC: 2.5.1.18] (inferred from 100% identity to sme:SM_b20005)

Predicted SEED Role

"Glutathione S-transferase, unnamed subgroup (EC 2.5.1.18)" in subsystem Glutathione: Non-redox reactions (EC 2.5.1.18)

MetaCyc Pathways

• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• pentachlorophenol degradation (3/10 steps found)
• gliotoxin biosynthesis (2/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18

Sequence Analysis Tools

See Q92XF3 at UniProt or InterPro

Protein Sequence (203 amino acids)

>SM_b20005 glutathione S-transferase (Sinorhizobium meliloti 1021)
MKLYHHPLSGHAHRARLFLSLLGIEHELVVVDFARREHKQADFLKLNSFGQVPVLDDAGT
IISDSNAILVYLAKKTGRPDWLPEDAVGAAAVQRWLSVAAGQIAHGPAQARLINVFKAPY
RPEEVIPRSHAILALIEQELEGRGWIAADRPTIADVALYSYVARAPEGDVDLQPYPEIRA
WLARIEALPGFVEFEKTPVGLAA