Protein Info for SGL_RS15335 in Synechocystis sp000284455 PCC 6803

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 603 TIGR00418: threonine--tRNA ligase" amino acids 26 to 596 (571 residues), 792.1 bits, see alignment E=1.9e-242 PF07973: tRNA_SAD" amino acids 121 to 173 (53 residues), 43.2 bits, see alignment 4.6e-15 PF00587: tRNA-synt_2b" amino acids 287 to 494 (208 residues), 152 bits, see alignment E=2.8e-48 PF03129: HGTP_anticodon" amino acids 506 to 595 (90 residues), 77.7 bits, see alignment E=9.2e-26

Best Hits

Swiss-Prot: 100% identical to SYT_SYNY3: Threonine--tRNA ligase (thrS) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to syn:sll0078)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (603 amino acids)

>SGL_RS15335 threonine--tRNA ligase (Synechocystis sp000284455 PCC 6803)
MAKTVVSPDTIALPRTSESEQLKKIRHTTSHVMAMAVQKLFPKAQVTIGPWTETGFYYDF
DVAEPFTEADLKAIKKEMVKIINKKLPVIREEISREEAKQRIESIQEPYKLEILDSIHEP
ITVYHLGDQWWDLCAGPHLESTADINPKAIALESVAGAYWRGDANKAQLQRIYGTAWETP
EQLAEYQRRKEEALKRDHRKLGKELGLFIFADPVGPGLPLWTPKGTIIRTILEDFLKQEQ
IKRGYLPVVTPHIARVDLFKQSGHWQKYQEDMFPMMAESPEEAAQEMGFVLKPMNCPFHI
QIYKSELRSYRDLPLRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPDQLDEEFL
SVVDLILTVFKSLQLKNFKARLSFRDPESDKYIGSDEAWEKAQGAIRRAVQELEMDYFEA
PGEAAFYGPKLDFIFQDALEREWQLGTVQVDYNLPERFDLEYIAADGSRQRPVMIHRAPF
GSLERLIGILIEEYAGDFPLWLAPIQVRLLAVSDEFLPFAQSIVQQMQSLGLRAEVDTSG
DRLGKMIRNAEKQKIPVMAVIGAKEVEANALNIRTRASGELGEIPVTEVLEKLQATVTNH
DTW