Protein Info for SGL_RS14710 in Synechocystis sp000284455 PCC 6803

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 13 to 1194 (1182 residues), 1781.1 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 17 to 307 (291 residues), 277.1 bits, E=6.1e-86
• PF00809: Pterin_bind
  • amino acids 344 to 575 (232 residues), 174.8 bits, E=6.5e-55
• PF02607: B12-binding_2
  • amino acids 638 to 711 (74 residues), 75.8 bits, 6.2e-25
• PF02310: B12-binding
  • amino acids 726 to 842 (117 residues), 66.8 bits, E=4.2e-22
• PF02965: Met_synt_B12
  • amino acids 937 to 1194 (258 residues), 120.7 bits, E=2e-38

Best Hits

Swiss-Prot: 100% identical to METH_SYNY3: Methionine synthase (metH) from Synechocystis sp. (strain PCC 6803 / Kazusa)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (19/25 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• L-methionine salvage from L-homocysteine (2/3 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (8/12 steps found)
• folate transformations II (plants) (7/11 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• folate transformations I (8/13 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (5/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (5/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (4/8 steps found)
• L-methionine biosynthesis III (1/4 steps found)
• L-methionine biosynthesis I (1/5 steps found)
• superpathway of L-methionine salvage and degradation (6/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (1195 amino acids)

>SGL_RS14710 methionine synthase (Synechocystis sp000284455 PCC 6803)
MKSAFLDRIHSPDRPVLVFDGAMGTNLQVQNLTAADFGGAEYEGCNEYLVHTKPEAVATV
HRAFYEAGADVVETDTFGGTPLVLAEYDLADQSYYLNKAAAELAKAVAAEFSTPEKPRFV
AGSMGPGTKLPTLGHVDYDSLKDAYVVQVRGLYDGGVDLLLVETCQDVLQIKAALNAIEQ
VFAEKGDRLPLMVSVTMETMGTMLVGTEMAAALAILEPYPIDILGLNCATGPDLMKEHVK
YLSEHSPFVVSCIPNAGLPENVGGQAFYRLTPMELQMSLMHFIEDLGVQVIGGCCGTRPD
HIKALADIAKDLQPKQRQPHYEPSAASIYSTQTYAQENSFLIIGERLNASGSKKCRDLLN
AEDWDSLVSLAKSQVKEGAQILDVNVDYVGRDGVRDMKELASRLVNNVTLPLMLDSTEWQ
KMEAGLKVAGGKCILNSTNYEDGEERFYKVLEIAKEYGAGIVIGTIDEDGMGRTADKKFE
IAKRAYEAAIAFGIPATEIFFDPLALPISTGIEEDRENGKATVDAIRRIRQELPDCHILL
GVSNVSFGLNPAARQVLNSIFLHECMQVGMDAAIVSANKILPLAKIDPEQQQVCLDLIYD
RREFEGERCTYDPLTKLTTLFEGKTTKRDKSGDANLPVEERLKRHIIDGERLGLEEALNE
ALKLYAPLDIINIYLLDGMKVVGELFGSGQMQLPFVLQSAQTMKAAVAFLEPHMDKDDSA
DNAKGTFLIATVKGDVHDIGKNLVDIILSNNGYRVVNLGIKQPVENIIEAYKKHRPDCIA
MSGLLVKSTAFMKENLEVFNQEGITVPVILGGAALTPKFVHQDCQNTYKGQVIYGKDAFA
DLHFMDKLMPAKNSHNWDDFQGFLGEYATENGHNVTTDDGAKTNFGIEEEKLIDASEQSR
EPEVIDTVRSEAVDPDLERPVPPFWGTKILQSSDISLDEVFPLLDLQALFVGQWQFRKPR
EQSREEYEQFLAEKVHPILAEWKGKVMAENLLHPTVVYGYFPCQSQGNTLLIYDPELVSQ
NNGQIPPDATAIAKFEFPRQKSGRRLCIADFFASKESGITDVFPLQAVTVGEIATEYARK
LFAGDNYTDYLYFHGMAVQMAEALAEWTHQRIRQELGFGHLDPDNIRDLLQQRYQGSRYS
FGYPACPNMQDQYTQLELLQTERIGLYMDESEQVYPEQSTTAIISYHPAAKYFSA