Protein Info for SGL_RS14350 in Synechocystis sp000284455 PCC 6803
Annotation: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 100% identity to syn:slr0646)Predicted SEED Role
"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)
MetaCyc Pathways
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan maturation (meso-diaminopimelate containing) (4/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-
Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (486 amino acids)
>SGL_RS14350 D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (Synechocystis sp000284455 PCC 6803) MALVLLSQWKKYLDPLPLQLTGTGIFYLCLTTGVGAQADRLCVGDLQQKLESHLQQPELS RAQWGIAIQPLTDSQPIYQYQADRFFIPASNQKLITTAMALQELGPNFRFTTQVYQRDGG KKVQVISSGDPSFDVDDLTAIAKGLKDRGVTAIEELELVDTIAPQDYQRPSWEWDDLHYG YAPPVNGAILTGNQVILTLTPQNLGEPLTPTWSDPIAGAQWQIINQTETAVDPHQPPQVQ QIFGQRQLVITGGLPPAGETRSITLAVADPQQYFLASLQQKLAEQGISVSTSIVSANTKA IAPAPLLALTSPPLWTLIKTVNQDSNNLYAEALLNAIQPPSQATDWQSYVERLGLATTTV RLRDGSGLSRQDLVTPQALVQLLINQTQKSTGTIYQQSLAVAGRSGTLERSFADTPLVGK MRGKTGTLTGVVSLTGYVENQQWGTVAFSFMVNNSDLGASVLREAMKQMVLWTAQVEKCQ PSDQGR