Protein Info for SGL_RS13500 in Synechocystis sp000284455 PCC 6803

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 831 PF17191: RecG_wedge" amino acids 146 to 311 (166 residues), 83.9 bits, see alignment E=3.5e-27 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 155 to 797 (643 residues), 874 bits, see alignment E=3.7e-267 PF01336: tRNA_anti-codon" amino acids 189 to 276 (88 residues), 34.1 bits, see alignment E=7.6e-12 PF04851: ResIII" amino acids 405 to 563 (159 residues), 47.2 bits, see alignment E=8.7e-16 PF00270: DEAD" amino acids 407 to 568 (162 residues), 88.4 bits, see alignment E=1.6e-28 PF00271: Helicase_C" amino acids 606 to 720 (115 residues), 68.9 bits, see alignment E=1.5e-22 PF19833: RecG_dom3_C" amino acids 749 to 809 (61 residues), 47.1 bits, see alignment 7.3e-16

Best Hits

Swiss-Prot: 100% identical to RECG_SYNY3: ATP-dependent DNA helicase RecG (recG) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to syn:slr0020)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (831 amino acids)

>SGL_RS13500 ATP-dependent DNA helicase RecG (Synechocystis sp000284455 PCC 6803)
MQCSLVEVSVSVDWPRLQKALTVEVERGFQNLQGKQHRFGDFLCLSFGSPPPPGSSPGDR
QKWREFAQRFAQYDQLEEAERKSLVASTRRFLHQLRRSLESPPRDSVPKKDLLAQVNRPQ
VSEPRYPKSGQIELHTPLATVVSQSQHQTKLLKNLGLATVEDLLFYFPRDYLDYAQQVTI
AELTAGETVTIVGRVVNCTCFTSPKNQNLNILQIQLRDQTGRIKLSRFYAGKRFAHRGWQ
EKIKKLYPPQAVVAASGLVKSSKFGLTLDNPEIEVLDRHSPSIDSFKVGRVLPVYPLTEG
ITADFLRKLVLACQTAIAKLSDPLPQEIREKYELIDLQTAIAQIHFPENTEKLSLARRRL
VFDEFFYLQLGFLQRRYEQKQQQQSAIFTPHGELLEKFSDLLPFRLTQAQQRVVNEILQD
LNKPSPMNRLVQGDVGSGKTVVGVFAILAALQGGYQAALMAPTEVLAEQHYQKLVSWFNL
LYLPVELLTGSTKTAKRREIHAQLSTGQLPLLVGTHALIQETVNFQRLGLVVIDEQHRFG
VQQRAKLLAKGNAPHVLSMTATPIPRTLALTLHGDLEVSQIDELPPGRQPIHTSVITAKE
RPQMYELIRREVAQGRQVYIIFPAIEESEKLDIKAAVEEHKYLTEKIFPNFNIGLLHGRL
KSAEKEAALTAFREKQTEIIVSTTVIEVGVDVPNATVMVIENAERFGLSQLHQLRGRVGR
GSHQSYCLLVTNSKSNDARQRLGVMEQSQDGFFIAEMDLRLRGPGEFLGTKQSGLPDFAL
ASLVEDQEVLLLAREAAETMMVEDPNLEAHPDLKIKLVQRYEKLLGGEILT