Protein Info for SGL_RS10950 in Synechocystis sp000284455 PCC 6803

Annotation: phosphoribosylamine--glycine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 PF02844: GARS_N" amino acids 1 to 102 (102 residues), 117.9 bits, see alignment E=7.7e-38 TIGR00877: phosphoribosylamine--glycine ligase" amino acids 1 to 419 (419 residues), 585.2 bits, see alignment E=3.6e-180 PF01071: GARS_A" amino acids 103 to 291 (189 residues), 261.3 bits, see alignment E=1.5e-81 PF02786: CPSase_L_D2" amino acids 107 to 187 (81 residues), 20 bits, see alignment E=1.1e-07 PF02655: ATP-grasp_3" amino acids 131 to 286 (156 residues), 26.8 bits, see alignment E=1.3e-09 PF02843: GARS_C" amino acids 327 to 417 (91 residues), 126.1 bits, see alignment E=1.4e-40

Best Hits

Swiss-Prot: 100% identical to PUR2_SYNY3: Phosphoribosylamine--glycine ligase (purD) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 100% identity to syn:slr1159)

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (419 amino acids)

>SGL_RS10950 phosphoribosylamine--glycine ligase (Synechocystis sp000284455 PCC 6803)
MKVAVIGSGGREHTLAWSLVRSPEVSHCYCLPGNGGTAGLPKTENVAIAVDQLGEICEFC
QTEKINLVVVGPELPLTLGLTDQLQALGIKVFGPTKAGAELEASKSWTKQLLIEAGVPTA
FGETFTEPAPAQAYATKMGAPIVVKADGLAAGKGVIVAQTSAEATTAIAELFDQGFEKIV
VEEFLPGEEVSVLALCDGKTVIPLLPAQDHKRIGEGDQGLNTGGMGAYCPAPIAPPAVID
QVQKQILQPTANALAKRGIDYRGVLYAGLMVSPTGEIKVLEYNCRFGDPETQAVLPLLAT
PLEKVLMACVEQNLEQLGPLQWHSGNAVCVVVAAGGYPGSYRKGDEINGLAEAEAQDVKV
FHAGTELKEGKVLTNGGRVLGVTALGKDLSTAIATAYRGVEQIDFDGMYYRRDIGHKAL