Protein Info for SGL_RS10240 in Synechocystis sp000284455 PCC 6803

Annotation: bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase" amino acids 1 to 200 (200 residues), 276.3 bits, see alignment E=1.3e-86 PF00926: DHBP_synthase" amino acids 7 to 199 (193 residues), 287 bits, see alignment E=6e-90 PF00925: GTP_cyclohydro2" amino acids 211 to 376 (166 residues), 247.1 bits, see alignment E=6.8e-78 TIGR00505: GTP cyclohydrolase II" amino acids 214 to 400 (187 residues), 257.1 bits, see alignment E=8.1e-81

Best Hits

Swiss-Prot: 100% identical to RIBBA_SYNY3: Riboflavin biosynthesis protein RibBA (ribBA) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 100% identity to syn:sll1894)

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (556 amino acids)

>SGL_RS10240 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II (Synechocystis sp000284455 PCC 6803)
MFDAIDAALADIKAGKAVVVVDDENRENEGDLICAAQFATPALVNFMAVEARGLICLAMT
GDRLDELDLPLMVSKNTDSNQTAFTVSIDAAPHLGVTTGISAEDRARTIQIAINPVTRPE
DLSRPGHIFPLRAKTGGVLKRAGHTEAAVDLSRLAGLYPAGVICEIQNADGSMARLPELV
EYARKYDLKLISIADLISYRLQHDRFVQRETICEFPSQFGEFKLYAYRNLLDQTEHIAIV
KGDPSEFGQQPVMVRMHSECLTGDALGSLRCDCRMQLQAALKMLENHGLGVVVYLRQEGR
GIGLVNKLKAYSLQDLGYDTVEANERLGFPADLRDYGMGAQMLNDLGVKQIRLITNNPRK
IAGLKGYGLEIVERVPLLIEANDYNSHYLTTKAEKLGHWLVKNYLLAIAIKFTPNVASAQ
QRYEKLEKIRALLQGTPLMVHEDNRPVAIALFGKNSLIFHVGLDQNLPSGQPWQKHDHSP
YPALVKDFLVQLKQWPDLQALAFLIAQGKDPMETLQVSLDRENVSFADLTADALNQWQPQ
TVYTYSKQGSGEMTNR