Protein Info for SGL_RS10075 in Synechocystis sp000284455 PCC 6803

Annotation: A-adding tRNA nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 942 PF00571: CBS" amino acids 331 to 385 (55 residues), 45.2 bits, see alignment 9.5e-16 amino acids 394 to 447 (54 residues), 49.6 bits, see alignment 4.1e-17 PF01743: PolyA_pol" amino acids 512 to 663 (152 residues), 98.4 bits, see alignment E=4.1e-32

Best Hits

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 100% identity to syn:sll1253)

Predicted SEED Role

"tRNA nucleotidyltransferase, A-adding (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.25)

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.19, 2.7.7.25

Use Curated BLAST to search for 2.7.7.19 or 2.7.7.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (942 amino acids)

>SGL_RS10075 A-adding tRNA nucleotidyltransferase (Synechocystis sp000284455 PCC 6803)
MDLILCHQTADFDVLGAAVGLAKLHPGSRIVLTGGSHPTVRQFLALHRNEFPLIELRSVN
PDKIRSLYIVDNQQGDRLGKAADWLTLPHLRQVAIYDHHLNSPRDIEADIWELEAVGAST
TLIVEKLQRADISLSMVEASVMALGIHVDTGSLTFTQTTVRDVKALAWLMEQGANLRLIA
EYADPGFPPPLQFLFAEAMQNLHKEMVRGYWLGSVLLTTENFVPGLSHLTERLLSLTECD
ALLLGHVYDKGKDKTKNDEQREKTGVISNQRFSLIGRTRIPDTDLTQLLEPYGGGGHAQA
AAVNLRDVEPTTVMAEIYQALQRQIPKPLLARDFMSSPVRTIRPHTTIEQAQRVLFRYGH
SGLTVVNQEEKLVGIISRRDLDLALHHGFSHAPVKGYMTRNVKTIAPDTPLPRIEAIMVA
DDVGRLPVMDQEKLVGIVTRTDVLRQLLQDKQEQSGRFGAPRSGSLRDRPTTPNQNFGQG
SLLQLLKTHLPPATWQLLTTAAQQAQTRGWHLYLVGGAVRDLWLRHSQGAEKHQNITFQD
IDLVVDGFHATADVGAGVELAQALQGDYPGARLSVHGEFQTAALLWHKDPQLDTLWVDIA
TARTEFYPYPAANPEVEASSIRQDLYRRDFTINALSIRLTNPNPGKLLDFFGGMNDLQSQ
QVRVLHANSFIEDPTRIYRAVRFVVRLGFELEPQTETYIRYAIASGVYERWRLTEHPTPA
LTTRLKAELSIILKAPYWKGALTLLADLDALKCLHAELKLTEQLWWQVRYLSRWLRWFDP
ERNLEHWLLRLGILLGALPPQEREKIAAGLQLPKATIDGLTNLETIETAIAKGLNSPASP
KSSPKSSVIYQTLKDYDRFSLFLVAARGNKLLRKQIWFYFSQLCQVSPFLTGHDLKALGY
KPGPQFKQILTDLLNACLDGELGDRQQEEAWLRTHYPLPNKV