Protein Info for SGL_RS08000 in Synechocystis sp000284455 PCC 6803

Annotation: endonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 TIGR01083: endonuclease III" amino acids 12 to 204 (193 residues), 282.4 bits, see alignment E=8.1e-89 PF00730: HhH-GPD" amino acids 43 to 179 (137 residues), 97.3 bits, see alignment E=1e-31 PF00633: HHH" amino acids 108 to 137 (30 residues), 28.6 bits, see alignment 1.3e-10 PF10576: EndIII_4Fe-2S" amino acids 197 to 213 (17 residues), 20.1 bits, see alignment (E = 9.6e-08)

Best Hits

Swiss-Prot: 100% identical to END3_SYNY3: Endonuclease III (nth) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K10773, endonuclease III [EC: 4.2.99.18] (inferred from 100% identity to syn:slr1822)

Predicted SEED Role

"Endonuclease III (EC 4.2.99.18)" in subsystem Control of cell elongation - division cycle in Bacilli or DNA Repair Base Excision (EC 4.2.99.18)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (219 amino acids)

>SGL_RS08000 endonuclease III (Synechocystis sp000284455 PCC 6803)
MSSLLRKMASKKQRATEILLILKKLYPGATCSLDYQTPVQLLVATILSAQCTDERVNKVT
PALFQRYPDANALAYGDRQEIEELIHSTGFFRNKAKNIQGACRKIVEEFDGEVPQRMEEL
LTLPGVARKTANVVLAHAFGILAGVTVDTHVKRLSQRLGLTKATDPIRIERDLMKLIPQP
DWENFSIHIIYHGRAVCAARKPLCGECQLAHLCPSAQAS