Protein Info for SGL_RS07160 in Synechocystis sp000284455 PCC 6803

Annotation: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to GATA_SYNY3: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 100% identity to syn:slr0877)

MetaCyc: 74% identical to glutamyl-tRNA(Gln) amidotransferase, A subunit (Synechococcus elongatus PCC 7942 = FACHB-805)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]; Asparaginyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7, 6.3.5.6]

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
L-glutamate and L-glutamine biosynthesis (6/7 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)
L-citrulline biosynthesis (5/8 steps found)
superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (483 amino acids)

>SGL_RS07160 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA (Synechocystis sp000284455 PCC 6803)
MSLIRALHQQLIDQEISAVAVAQASLARIEAVDDRLKSFLQVTAPQAIAQAEKVDAQIAA
GEPIGLLAGIPIGIKDNLCTKGIVTTCASQILRGFVPPYESTVTEKLQKAGAVMVGKTNL
DEFAMGSSTENSGYQVTANPWDLTRVPGGSSGGSAAAVAADECLIALGSDTGGSIRQPAS
LCGVVGMKPTYGLVSRFGLVAYASSLDQIGPFARRVEDAAILLQAIAGHDSRDSTSLDVP
IPDYTQALKPDLKGVKVGVIMDAFGEGLDDTVNEAVQTAIAKLRELGATIEEIHCPRFRS
GIAAYYVIAPSEASANLARYDAVRYGLRAEADNLMEMYTHTRAKGFGAEVKRRIMLGTYA
LSAGYYDAYYLKAQKVRTLIKQDFDQAFTKVDVLVCPTAPTTAFKAGEKTDDPLSMYLSD
LMTIPVNLAGLPAMSVPCGFDDNNLPIGLQLVGNVLGEEMLFRVGYAYEQATTWHDRQPD
LSA