Protein Info for Rv3825c in Mycobacterium tuberculosis H37Rv

Annotation: Polyketide synthase Pks2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 200 400 600 800 1000 1200 1400 1600 1800 2000 2126 PF00109: ketoacyl-synt" amino acids 26 to 274 (249 residues), 328.1 bits, see alignment E=2.7e-101 PF00108: Thiolase_N" amino acids 186 to 227 (42 residues), 29.3 bits, see alignment (E = 3.8e-10) PF02801: Ketoacyl-synt_C" amino acids 282 to 395 (114 residues), 119.9 bits, see alignment 4e-38 PF16197: KAsynt_C_assoc" amino acids 407 to 513 (107 residues), 53.4 bits, see alignment 2.1e-17 PF00698: Acyl_transf_1" amino acids 553 to 869 (317 residues), 340.4 bits, see alignment E=8.4e-105 PF21089: PKS_DH_N" amino acids 914 to 1010 (97 residues), 79 bits, see alignment 1.6e-25 PF14765: PS-DH" amino acids 1044 to 1188 (145 residues), 87.8 bits, see alignment 4.1e-28 PF08240: ADH_N" amino acids 1453 to 1518 (66 residues), 24.6 bits, see alignment (E = 1.2e-08) PF00107: ADH_zinc_N" amino acids 1577 to 1694 (118 residues), 81.2 bits, see alignment 4.5e-26 PF13602: ADH_zinc_N_2" amino acids 1610 to 1748 (139 residues), 76.1 bits, see alignment 3.6e-24 PF08659: KR" amino acids 1772 to 1950 (179 residues), 199.7 bits, see alignment E=2.5e-62 PF00106: adh_short" amino acids 1775 to 1929 (155 residues), 33.4 bits, see alignment 2.1e-11 PF00550: PP-binding" amino acids 2045 to 2109 (65 residues), 36.5 bits, see alignment (E = 3e-12)

Best Hits

Swiss-Prot: 100% identical to PHAS_MYCBO: Phthioceranic/hydroxyphthioceranic acid synthase (pks2) from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

KEGG orthology group: K12431, polyketide synthase 2 (inferred from 100% identity to mbb:BCG_3888c)

MetaCyc: 100% identical to phthioceranic/hydroxyphthioceranic acid synthase monomer (Mycobacterium tuberculosis H37Rv)
RXN-17202 [EC: 2.3.1.287]; 2.3.1.287 [EC: 2.3.1.287]; 2.3.1.287 [EC: 2.3.1.287]; 2.3.1.287 [EC: 2.3.1.287]

Predicted SEED Role

"Polyketide synthase Pks2"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.287

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (2126 amino acids)

>Rv3825c Polyketide synthase Pks2 (Mycobacterium tuberculosis H37Rv)
LGLGSAASGTGADRGAWTLAEPRVTPVAVIGMACRLPGGIDSPELLWKALLRGDDLITEV
PPDRWDCDEFYDPQPGVPGRTVCKWGGFLDNPADFDCEFFGIGEREAIAIDPQQRLLLET
SWEAMEHAGLTQQTLAGSATGVFAGVTHGDYTMVAADAKQLEEPYGYLGNSFSMASGRVA
YAMRLHGPAITVDTACSSGLTAVHMACRSLHEGESDVALAGGVALMLEPRKAAAGSALGM
LSPTGRCRAFDVAADGFVSGEGCAVVVLKRLPDALADGDRILAVIRGTSANQDGHTVNIA
TPSQPAQVAAYRAALAAGGVDAATVGMVEAHGPGTPIGDPIEYASVSEVYGVDGPCALAS
VKTNFGHTQSTAGVLGLIKVVLALKHGVVPRNLHFTRLPDEIAGITTNLFVPEVTTPWPT
NGRQVPRRAAVSSYGFSGTNVHAVVEQAPQTEAQPHAASTPPTGTPALFTLSASSADALR
QTAQRLTDWIQQHADSLVLSDLAYTLARRRTHRSVRTAVIASSVDELIAGLGEVADGDTV
YQPAVGQDDRGPVWLFSGQGSQWAAMGADLLTNESVFAATVAELEPLIAAESGFSVTEAM
TAPETVTGIDRVQPTIFAMQVALAATMAAYGVRPGAVIGHSMGESAAAVVAGVLSAEDGV
RVICRRSKLMATIAGSAAMASVELPALAVQSELTALGIDDVVVAVVTAPQSTVIAGGTES
VRKLVDIWERRDVLARAVAVDVASHSPQVDPILDELIAALADLNPKAPEIPYYSATLFDP
REAPACDARYWADNLRHTVRFSAAVRSALDDGYRVFAELSPHPLLTHAVDQIAGSVGMPV
AALAGMRREQPLPLGLRRLLTDLHNAGAAVDFSVLCPQGRLVDAPLPAWSHRFLFYDREG
VDNRSPGGSTVAVHPLLGAHVRLPEEPERHAWQADVGTATLPWLGDHRIHNVAALPGAAY
CEMALSAARAVLGEQSEVRDMRFEAMLLLDDQTPVSTVATVTSPGVVDFAVEALQEGVGH
HLRRASAVLQQVSGECEPPAYDMASLLEAHPCRVDGEDLRRQFDKHGVQYGPAFTGLAVA
YVAEDATATMLAEVALPGSIRSQQGLYAIHPALLDACFQSVGAHPDSQSVGSGLLVPLGV
RRVRAYAPVRTARYCYTRVTKVELVGVEADIDVLDAHGTVLLAVCGLRIGTGVSERDKHN
RVLNERLLTIEWHQRELPEMDPSGAGKWLLISDCAASDVTATRLADAFREHSAACTTMRW
PLHDDQLAAADQLRDQVGSDEFSGVVVLTGSNTGTPHQGSADRGAEYVRRLVGIARELSD
LPGAVPRMYVVTRGAQRVLADDCVNLEQGGLRGLLRTIGAEHPHLRATQIDVDEQTGVEQ
LARQLLATSEEDETAWRDNEWYVARLCPTPLRPQERRTIVADHQQSGMRLQIRTPGDMQT
IELAAFHRVPPGPGQIEVAVRASSVNFADVLIAFGRYPSFEGHLPQLGTDFAGVVTAVGP
GVTDHKVGDHVGGMSPNGCWGTFVTCDARLAATLPPGLGDAQAAAVTTAHATAWYGLHEL
ARIRAGDTVLIHSGTGGVGQAAIAIARAAGAEIFATAGTPQRRELLRNMGIEHVYDSRSI
EFAEQIRRDTNGRGVDVVLNSVTGAAQLAGLKLLAFRGRFVEIGKRDIYGDTKLGLFPFR
RNLSFYAVDLGLLSATHPEELRDLLGTVYRLTAAGELPMPQSTHYPLVEAATAIRVMGNA
EHTGKLVLHIPQTGKSLVTLPPEQAQVFRPDGSYIITGGLGGLGLFLAEKMAAAGCGRIV
LNSRTQPTQKMRETIEAIAAMGSEVVVECGDIAQPGTAERLVATAVATGLPVRGVLHAAA
VVEDATLANITDELLARDWAPKVHGAWELHEATSGQPLDWFCLFSSAAALTGSPGQSAYS
AANSWLDAFAHWRQAQGLPATAIAWGAWSDIGQLGWWSASPARASALEESNYTAITPDEG
AYAFEALLRHNRVYTGYAPVIGAPWLVAFAERSRFFEVFSSSNGSGTSKFRVELNELPRD
EWPARLRQLVAEQVSLILRRTVDPDRPLPEYGLDSLGALELRTRIETETGIRLAPKNVSA
TVRGLADHLYEQLAPDDAPAAALSSQ