Protein Info for Rv3543c in Mycobacterium tuberculosis H37Rv
Annotation: Probable acyl-CoA dehydrogenase FadE29
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to CHSE2_MYCTU: Acyl-CoA dehydrogenase FadE29 (fadE29) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 100% identity to mtc:MT3647)MetaCyc: 100% identical to 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit (Mycobacterium tuberculosis H37Rv)
1.3.99.-
Predicted SEED Role
"Probable acyl-CoA dehydrogenase FadE29 (EC 1.3.99.-); Acyl-CoA dehydrogenase IgrC" (EC 1.3.99.-)
MetaCyc Pathways
- superpathway of cholesterol degradation I (cholesterol oxidase) (34/42 steps found)
- superpathway of cholesterol degradation II (cholesterol dehydrogenase) (36/47 steps found)
- cholesterol degradation to androstenedione I (cholesterol oxidase) (12/17 steps found)
- cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (14/22 steps found)
- sitosterol degradation to androstenedione (8/18 steps found)
- cholesterol degradation to androstenedione III (anaerobic) (10/22 steps found)
- superpathway of cholesterol degradation III (oxidase) (23/49 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Brassinosteroid biosynthesis
- Caprolactam degradation
- Fatty acid metabolism
- Geraniol degradation
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.-
Use Curated BLAST to search for 1.3.99.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (387 amino acids)
>Rv3543c Probable acyl-CoA dehydrogenase FadE29 (Mycobacterium tuberculosis H37Rv) MFIDLTPEQRQLQAEIRQYFSNLISPDERTEMEKDRHGPAYRAVIRRMGRDGRLGVGWPK EFGGLGFGPIEQQIFVNEAHRADVPLPAVTLQTVGPTLQAHGSELQKKKFLPAILAGEAH FAIGYTEPEAGTDLASLRTTAVRDGDHYIVNGQKVFTTGAHDADYIWLACRTDPNAAKHK GISILIVDTKDPGYSWTPIILADGAHHTNATYYNDVRVPVDMLVGKENDGWRLITTQLNN ERVMLGPAGRFASIYDRVHAWASVPGGNGVTPIDHDDVKRALGEIRAIWRINELLNWQVA SAGEDINMADAAATKVFGTERVQRAGRLAEEIVGKYGNPAEPDTAELLRWLDAQTKRNLV ITFGGGVNEVMREMIAASGLKVPRVPR