Protein Info for Rv3537 in Mycobacterium tuberculosis H37Rv

Annotation: Probable dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01494: FAD_binding_3
  • amino acids 6 to 93 (88 residues), 25.1 bits, E=2.6e-09
• PF00890: FAD_binding_2
  • amino acids 7 to 541 (535 residues), 291.7 bits, E=2.9e-90
• PF01266: DAO
  • amino acids 7 to 52 (46 residues), 36.1 bits, 1.4e-12
• PF12831: FAD_oxidored
  • amino acids 7 to 82 (76 residues), 43.2 bits, E=8.3e-15
• PF13450: NAD_binding_8
  • amino acids 10 to 45 (36 residues), 27.4 bits, 8.4e-10

Best Hits

Swiss-Prot: 100% identical to 3O1D1_MYCTU: 3-oxosteroid 1-dehydrogenase (kstD) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: None (inferred from 100% identity to mbt:JTY_3602)

MetaCyc: 100% identical to 3-oxosteroid 1-dehydrogenase (Mycobacterium tuberculosis H37Rv)
3-oxosteroid 1-dehydrogenase. [EC: 1.3.99.4]; 1.3.99.4 [EC: 1.3.99.4]; 1.3.99.4 [EC: 1.3.99.4]

Predicted SEED Role

"3-oxosteroid 1-dehydrogenase (EC 1.3.99.4)" (EC 1.3.99.4)

MetaCyc Pathways

• androstenedione degradation I (aerobic) (22/25 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (34/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (36/47 steps found)
• superpathway of testosterone and androsterone degradation (22/28 steps found)
• testosterone degradation (anaerobic) (1/6 steps found)
• androstenedione degradation II (anaerobic) (13/27 steps found)
• superpathway of cholesterol degradation III (oxidase) (23/49 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.99.4

Protein Sequence (563 amino acids)

>Rv3537 Probable dehydrogenase (Mycobacterium tuberculosis H37Rv)
MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYGGSTARSGGGVWIPNNEVLK
RRGVRDTPEAARTYLHGIVGEIVEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYY
PEAPGGRPGGRSIEPKPFNARKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRG
VLRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVS
GVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPITTEWTVGASANTGD
GILAAEKLGAALDLMDDAWWGPTVPLVGKPWFALSERNSPGSIIVNMSGKRFMNESMPYV
EACHHMYGGEHGQGPGPGENIPAWLVFDQRYRDRYIFAGLQPGQRIPSRWLDSGVIVQAD
TLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPNLGEV
GHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNVSAPVMGHTYPGPGG
TIGPAMTFGYLAALHIADQAGKR