Protein Info for Rv3526 in Mycobacterium tuberculosis H37Rv

Annotation: Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to KSHA_MYCTU: 3-ketosteroid-9-alpha-monooxygenase, oxygenase component (kshA) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: None (inferred from 100% identity to mtb:TBMG_03565)

MetaCyc: 100% identical to 3-ketosteroid 9alpha-hydroxylase oxygenase component monomer (Mycobacterium tuberculosis H37Rv)
RXN-12691 [EC: 1.14.15.30]; 1.14.15.30 [EC: 1.14.15.30]; 1.14.15.30 [EC: 1.14.15.30]; 1.14.15.30 [EC: 1.14.15.30]

Predicted SEED Role

"Rieske (2Fe-2S) domain-containing protein"

MetaCyc Pathways

androstenedione degradation I (aerobic) (22/25 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (34/42 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (36/47 steps found)
superpathway of testosterone and androsterone degradation (22/28 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.15.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (386 amino acids)

>Rv3526 Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA (Mycobacterium tuberculosis H37Rv)
VSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDL
KVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTD
VRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMA
HFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMIN
WLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKG
FLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRA
NEFWNAEVEKNLKSREVSDDVPAEQH