Protein Info for Rv3401 in Mycobacterium tuberculosis H37Rv

Annotation: Conserved protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 786 PF03636: Glyco_hydro_65N" amino acids 15 to 270 (256 residues), 205.8 bits, see alignment E=1.2e-64 PF03632: Glyco_hydro_65m" amino acids 327 to 678 (352 residues), 465.1 bits, see alignment E=2.4e-143 PF03633: Glyco_hydro_65C" amino acids 687 to 750 (64 residues), 58.8 bits, see alignment E=5.2e-20

Best Hits

Swiss-Prot: 100% identical to Y3401_MYCTU: Uncharacterized glycosyl hydrolase Rv3401 (Rv3401) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: None (inferred from 100% identity to mbo:Mb3434)

Predicted SEED Role

"Maltose phosphorylase (EC 2.4.1.8) / Trehalose phosphorylase (EC 2.4.1.64)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis or Trehalose Uptake and Utilization (EC 2.4.1.64, EC 2.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.64 or 2.4.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (786 amino acids)

>Rv3401 Conserved protein (Mycobacterium tuberculosis H37Rv)
MITEDAFPVEPWQVRETKLNLNLLAQSESLFALSNGHIGLRGNLDEGEPFGLPGTYLNSF
YEIRPLPYAEAGYGYPEAGQTVVDVTNGKIFRLLVGDEPFDVRYGELISHERILDLRAGT
LTRRAHWRSPAGKQVKVTSTRLVSLAHRSVAAIEYVVEAIEEFVRVTVQSELVTNEDVPE
TSADPRVSAILDRPLQAVEHERTERGALLMHRTRASALMMAAGMEHEVEVPGRVEITTDA
RPDLARTTVICGLRPGQKLRIVKYLAYGWSSLRSRPALRDQAAGALHGARYSGWQGLLDA
QRAYLDDFWDSADVEVEGDPECQQAVRFGLFHLLQASARAERRAIPSKGLTGTGYDGHAF
WDTEGFVLPVLTYTAPHAVADALRWRASTLDLAKERAAELGLEGAAFPWRTIRGQESSAY
WPAGTAAWHINADIAMAFERYRIVTGDGSLEEECGLAVLIETARLWLSLGHHDRHGVWHL
DGVTGPDEYTAVVRDNVFTNLMAAHNLHTAADACLRHPEAAEAMGVTTEEMAAWRDAADA
ANIPYDEELGVHQQCEGFTTLAEWDFEANTTYPLLLHEAYVRLYPAQVIKQADLVLAMQW
QSHAFTPEQKARNVDYYERRMVRDSSLSACTQAVMCAEVGHLELAHDYAYEAALIDLRDL
HRNTRDGLHMASLAGAWTALVVGFGGLRDDEGILSIDPQLPDGISRLRFRLRWRGFRLIV
DANHTDVTFILGDGPGTQLTMRHAGQDLTLHTDTPSTIAVRTRKPLLPPPPQPPGREPVH
RRALAR