Protein Info for Rv3392c in Mycobacterium tuberculosis H37Rv

Annotation: Cyclopropane-fatty-acyl-phospholipid synthase 1 CmaA1 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 287 PF02353: CMAS" amino acids 5 to 283 (279 residues), 420.8 bits, see alignment E=5.8e-130 PF13489: Methyltransf_23" amino acids 52 to 181 (130 residues), 38.5 bits, see alignment E=2.5e-13 PF08242: Methyltransf_12" amino acids 69 to 163 (95 residues), 35.2 bits, see alignment E=4.4e-12 PF08241: Methyltransf_11" amino acids 69 to 165 (97 residues), 33.3 bits, see alignment E=1.6e-11 PF13649: Methyltransf_25" amino acids 69 to 162 (94 residues), 43.7 bits, see alignment E=9.5e-15

Best Hits

Swiss-Prot: 100% identical to CMAS1_MYCTA: Cyclopropane mycolic acid synthase 1 (cmaA1) from Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 100% identity to mtc:MT3499)

MetaCyc: 74% identical to methoxy mycolic acid synthase 2 (Mycobacterium tuberculosis H37Rv)
Cyclopropane-fatty-acyl-phospholipid synthase. [EC: 2.1.1.79]; 2.1.1.79 [EC: 2.1.1.79]; 2.1.1.79 [EC: 2.1.1.79]; 2.1.1.79 [EC: 2.1.1.79]

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79)" (EC 2.1.1.79)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (287 amino acids)

>Rv3392c Cyclopropane-fatty-acyl-phospholipid synthase 1 CmaA1 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 1) (Mycobacterium tuberculosis H37Rv)
MPDELKPHFANVQAHYDLSDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKL
GLQPGMTLLDVGCGWGATMMRAVEKYDVNVVGLTLSKNQANHVQQLVANSENLRSKRVLL
AGWEQFDEPVDRIVSIGAFEHFGHERYDAFFSLAHRLLPADGVMLLHTITGLHPKEIHER
GLPMSFTFARFLKFIVTEIFPGGRLPSIPMVQECASANGFTVTRVQSLQPHYAKTLDLWS
AALQANKGQAIALQSEEVYERYMKYLTGCAEMFRIGYIDVNQFTCQK