Protein Info for Rv3370c in Mycobacterium tuberculosis H37Rv

Annotation: Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1079 TIGR00594: DNA polymerase III, alpha subunit" amino acids 47 to 915 (869 residues), 651.3 bits, see alignment E=1.6e-199 PF02811: PHP" amino acids 48 to 219 (172 residues), 111.3 bits, see alignment E=1.6e-35 PF07733: DNA_pol3_alpha" amino acids 338 to 582 (245 residues), 283.1 bits, see alignment E=6.2e-88 PF17657: DNA_pol3_finger" amino acids 585 to 749 (165 residues), 187.4 bits, see alignment E=3.2e-59 PF14579: HHH_6" amino acids 825 to 913 (89 residues), 80.5 bits, see alignment E=2.1e-26 PF01336: tRNA_anti-codon" amino acids 992 to 1064 (73 residues), 27.7 bits, see alignment 5.4e-10

Best Hits

Swiss-Prot: 100% identical to DNAE2_MYCTO: Error-prone DNA polymerase (dnaE2) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 73% identity to asd:AS9A_4011)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1079 amino acids)

>Rv3370c Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase) (Mycobacterium tuberculosis H37Rv)
MERVLNGKPRHAGVPAFDADGDVPRSRKRGAYQPPGRERVGSSVAYAELHAHSAYSFLDG
ASTPEELVEEAARLGLCALALTDHDGLYGAVRFAEAAAELDVRTVFGAELSLGATARTER
PDPPGPHLLVLARGPEGYRRLSRQLAAAHLAGGEKGKPRYDFDALTEAAGGHWHILTGCR
KGHVRQALSQGGPAAAQRALADLVDRFTPSRVSIELTHHGHPLDDERNAALAGLAPRFGV
GIVATTGAHFADPSRGRLAMAMAAIRARRSLDSAAGWLAPLGGAHLRSGEEMARLFAWCP
EAVTAAAELGERCAFGLQLIAPRLPPFDVPDGHTEDSWLRSLVMAGARERYGPPKSAPRA
YSQIEHELKVIAQLRFPGYFLVVHDITRFCRDNDILCQGRGSAANSAVCYALGVTAVDPV
ANELLFERFLSPARDGPPDIDIDIESDQREKVIQYVYHKYGRDYAAQVANVITYRGRSAV
RDMARALGFSPGQQDAWSKQVSHWTGQADDVDGIPEQVIDLATQIRNLPRHLGIHSGGMV
ICDRPIADVCPVEWARMANRSVLQWDKDDCAAIGLVKFDLLGLGMLSALHYAKDLVAEHK
GIEVDLARLDLSEPAVYEMLARADSVGVFQVESRAQMATLPRLKPRVFYDLVVEVALIRP
GPIQGGSVHPYIRRRNGVDPVIYEHPSMAPALRKTLGVPLFQEQLMQLAVDCAGFSAAEA
DQLRRAMGSKRSTERMRRLRGRFYDGMRALHGAPDEVIDRIYEKLEAFANFGFPESHALS
FASLVFYSAWFKLHHPAAFCAALLRAQPMGFYSPQSLVADARRHGVAVHGPCVNASLAHA
TCENAGTEVRLGLGAVRYLGAELAEKLVAERTANGPFTSLPDLTSRVQLSVPQVEALATA
GALGCFGMSRREALWAAGAAATGRPDRLPGVGSSSHIPALPGMSELELAAADVWATGVSP
DSYPTQFLRADLDAMGVLPAERLGSVSDGDRVLIAGAVTHRQRPATAQGVTFINLEDETG
MVNVLCTPGVWARHRKLAHTAPALLIRGQVQNASGAITVVAERMGRLTLAVGARSRDFR