Protein Info for Rv3262 in Mycobacterium tuberculosis H37Rv

Annotation: Probable F420 biosynthesis protein FbiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 372 to 392 (21 residues), see Phobius details TIGR01916: coenzyme F420-0:L-glutamate ligase" amino acids 12 to 242 (231 residues), 293.9 bits, see alignment E=9.2e-92 PF01996: F420_ligase" amino acids 19 to 219 (201 residues), 171.9 bits, see alignment E=1.4e-54 TIGR03553: F420 biosynthesis protein FbiB, C-terminal domain" amino acids 255 to 447 (193 residues), 342.4 bits, see alignment E=7.5e-107 PF00881: Nitroreductase" amino acids 260 to 428 (169 residues), 104.9 bits, see alignment E=5.5e-34

Best Hits

Swiss-Prot: 100% identical to FBIB_MYCBP: Coenzyme F420:L-glutamate ligase (fbiB) from Mycobacterium bovis (strain BCG / Pasteur 1173P2)

KEGG orthology group: K12234, coenzyme F420-0:L-glutamate ligase / coenzyme F420-1:gamma-L-glutamate ligase [EC: 6.3.2.31 6.3.2.34] (inferred from 100% identity to mtc:MT3361)

MetaCyc: 100% identical to dehydro coenzyme F420-0 reductase/coenzyme F420:L-glutamate ligase (Mycobacterium tuberculosis H37Rv)
RXN-8081 [EC: 6.3.2.34]; RXN-8080 [EC: 6.3.2.34, 6.3.2.31]; RXN-20703 [EC: 6.3.2.34, 6.3.2.31, 1.3.8.17]

Predicted SEED Role

"Coenzyme F420-0:L-glutamate ligase @ Coenzyme F420-1:L-glutamate ligase / domain of unknown function"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.17 or 6.3.2.31 or 6.3.2.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>Rv3262 Probable F420 biosynthesis protein FbiB (Mycobacterium tuberculosis H37Rv)
LTGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRL
VPAPEDPEQRDRLRRKLIEDEAVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELAL
LPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQTDAAVGAAGLAVLRNYAGVR
DPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDL
FWLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWL
QTPAIRARLLDRMKDKWRSDLTSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSY
PDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGSTIFAADLVRDELDLPVDWEP
LGAIAIGYADEPSGLRDPVPAADLLILK