Protein Info for Rv3248c in Mycobacterium tuberculosis H37Rv

Annotation: Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05221: AdoHcyase
  • amino acids 20 to 494 (475 residues), 493.7 bits, E=5.4e-152
• TIGR00936: adenosylhomocysteinase
  • amino acids 21 to 487 (467 residues), 689.1 bits, E=9.7e-212
• PF00670: AdoHcyase_NAD
  • amino acids 253 to 415 (163 residues), 260.6 bits, E=2e-81
• PF07991: IlvN
  • amino acids 273 to 338 (66 residues), 31 bits, E=5.5e-11
• PF02826: 2-Hacid_dh_C
  • amino acids 273 to 362 (90 residues), 37.3 bits, E=5.5e-13

Best Hits

Swiss-Prot: 100% identical to SAHH_MYCBP: Adenosylhomocysteinase (ahcY) from Mycobacterium bovis (strain BCG / Pasteur 1173P2)

KEGG orthology group: K01251, adenosylhomocysteinase [EC: 3.3.1.1] (inferred from 100% identity to mbo:Mb3276c)

Predicted SEED Role

"Adenosylhomocysteinase (EC 3.3.1.1)" in subsystem Methionine Biosynthesis or Methionine Degradation (EC 3.3.1.1)

MetaCyc Pathways

• S-adenosyl-L-methionine salvage II (3/3 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (4/5 steps found)
• L-methionine degradation I (to L-homocysteine) (2/3 steps found)
• superpathway of L-methionine salvage and degradation (10/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.3.1.1

Protein Sequence (495 amino acids)

>Rv3248c Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) (Mycobacterium tuberculosis H37Rv)
MTGNLVTKNSLTPDVRNGIDFKIADLSLADFGRKELRIAEHEMPGLMSLRREYAEVQPLK
GARISGSLHMTVQTAVLIETLTALGAEVRWASCNIFSTQDHAAAAVVVGPHGTPDEPKGV
PVFAWKGETLEEYWWAAEQMLTWPDPDKPANMILDDGGDATMLVLRGMQYEKAGVVPPAE
EDDPAEWKVFLNLLRTRFETDKDKWTKIAESVKGVTEETTTGVLRLYQFAAAGDLAFPAI
NVNDSVTKSKFDNKYGTRHSLIDGINRGTDALIGGKKVLICGYGDVGKGCAEAMKGQGAR
VSVTEIDPINALQAMMEGFDVVTVEEAIGDADIVVTATGNKDIIMLEHIKAMKDHAILGN
IGHFDNEIDMAGLERSGATRVNVKPQVDLWTFGDTGRSIIVLSEGRLLNLGNATGHPSFV
MSNSFANQTIAQIELWTKNDEYDNEVYRLPKHLDEKVARIHVEALGGHLTKLTKEQAEYL
GVDVEGPYKPDHYRY