Protein Info for Rv3106 in Mycobacterium tuberculosis H37Rv
Annotation: NADPH:adrenodoxin oxidoreductase FprA (NADPH-ferredoxin reductase)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to FPRA_MYCTO: NADPH-ferredoxin reductase FprA (fprA) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
KEGG orthology group: K00528, ferredoxin--NADP+ reductase [EC: 1.18.1.2] (inferred from 100% identity to mbo:Mb3133)Predicted SEED Role
"Ferredoxin--NADP(+) reductase, actinobacterial (eukaryote-like) type (EC 1.18.1.2)" (EC 1.18.1.2)
MetaCyc Pathways
- ethene biosynthesis V (engineered) (17/25 steps found)
- NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) (1/4 steps found)
- photosynthesis light reactions (1/4 steps found)
- oxygenic photosynthesis (10/17 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (17/27 steps found)
- superpathway of photosynthetic hydrogen production (1/6 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (15/26 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.18.1.2
Use Curated BLAST to search for 1.18.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (456 amino acids)
>Rv3106 NADPH:adrenodoxin oxidoreductase FprA (NADPH-ferredoxin reductase) (Mycobacterium tuberculosis H37Rv) MRPYYIAIVGSGPSAFFAAASLLKAADTTEDLDMAVDMLEMLPTPWGLVRSGVAPDHPKI KSISKQFEKTAEDPRFRFFGNVVVGEHVQPGELSERYDAVIYAVGAQSDRMLNIPGEDLP GSIAAVDFVGWYNAHPHFEQVSPDLSGARAVVIGNGNVALDVARILLTDPDVLARTDIAD HALESLRPRGIQEVVIVGRRGPLQAAFTTLELRELADLDGVDVVIDPAELDGITDEDAAA VGKVCKQNIKVLRGYADREPRPGHRRMVFRFLTSPIEIKGKRKVERIVLGRNELVSDGSG RVAAKDTGEREELPAQLVVRSVGYRGVPTPGLPFDDQSGTIPNVGGRINGSPNEYVVGWI KRGPTGVIGTNKKDAQDTVDTLIKNLGNAKEGAECKSFPEDHADQVADWLAARQPKLVTS AHWQVIDAFERAAGEPHGRPRVKLASLAELLRIGLG