Protein Info for Rv3051c in Mycobacterium tuberculosis H37Rv

Annotation: Ribonucleoside-diphosphate reductase (alpha chain) NrdE (ribonucleotide reductase small subunit) (R1F protein)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 PF08343: RNR_N" amino acids 1 to 76 (76 residues), 95.4 bits, see alignment E=2.5e-31 TIGR04170: ribonucleoside-diphosphate reductase, class 1b, alpha subunit" amino acids 1 to 693 (693 residues), 1259.6 bits, see alignment E=0 PF00317: Ribonuc_red_lgN" amino acids 78 to 151 (74 residues), 60.3 bits, see alignment E=2.4e-20 TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit" amino acids 82 to 675 (594 residues), 530.6 bits, see alignment E=5.2e-163 PF02867: Ribonuc_red_lgC" amino acids 155 to 673 (519 residues), 534.5 bits, see alignment E=4.4e-164

Best Hits

Swiss-Prot: 100% identical to RIR1_MYCTO: Ribonucleoside-diphosphate reductase subunit alpha (nrdE) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 88% identity to asd:AS9A_3657)

MetaCyc: 74% identical to ribonucleoside-diphosphate reductase 2 subunit alpha (Escherichia coli K-12 substr. MG1655)
Ribonucleoside-diphosphate reductase. [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]

Predicted SEED Role

"Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (693 amino acids)

>Rv3051c Ribonucleoside-diphosphate reductase (alpha chain) NrdE (ribonucleotide reductase small subunit) (R1F protein) (Mycobacterium tuberculosis H37Rv)
MLNLYDADGKIQFDKDREAAHQYFLQHVNQNTVFFHNQDEKLDYLIRENYYEREVLDQYS
RNFVKTLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGD
TALAELLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLLRVEDNMESIGRSINSALQLS
KRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDAFSYANQLGARQGAGAVYLHA
HHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERVYGVP
FADISVTEKYYEMVDDARIRKTKIKAREFFQTLAELQFESGYPYIMFEDTVNRANPIDGK
ITHSNLCSEILQVSTPSLFNEDLSYAKVGKDISCNLGSLNIAKTMDSPDFAQTIEVAIRA
LTAVSDQTHIKSVPSIEQGNNDSHAIGLGQMNLHGYLARERIFYGSDEGIDFTNIYFYTV
LYHALRASNRIAIERGTHFKGFERSKYASGEFFDKYTDQIWEPKTQKVRQLFADAGIRIP
TQDDWRRLKESVQAHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSKVEIRKEGKIGRVY
YPAPYMTNDNLEYYEDAYEIGYEKIIDTYAAATQHVDQGLSLTLFFKDTATTRDVNKAQI
YAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML