Protein Info for Rv3014c in Mycobacterium tuberculosis H37Rv

Annotation: DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+])

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 PF01653: DNA_ligase_aden" amino acids 13 to 328 (316 residues), 421.4 bits, see alignment E=8.9e-130 TIGR00575: DNA ligase, NAD-dependent" amino acids 20 to 678 (659 residues), 836.3 bits, see alignment E=8.8e-256 PF03120: DNA_ligase_OB" amino acids 331 to 408 (78 residues), 107.6 bits, see alignment E=9.8e-35 PF03119: DNA_ligase_ZBD" amino acids 417 to 444 (28 residues), 38.1 bits, see alignment (E = 4.6e-13) PF12826: HHH_2" amino acids 527 to 590 (64 residues), 80.2 bits, see alignment E=4.2e-26 PF14520: HHH_5" amino acids 532 to 577 (46 residues), 36.2 bits, see alignment 2.7e-12 PF00633: HHH" amino acids 551 to 579 (29 residues), 24.9 bits, see alignment (E = 5.4e-09) PF00533: BRCT" amino acids 609 to 682 (74 residues), 54.1 bits, see alignment E=5.8e-18 PF12738: PTCB-BRCT" amino acids 618 to 674 (57 residues), 33 bits, see alignment 1.9e-11

Best Hits

Swiss-Prot: 100% identical to DNLJ_MYCBP: DNA ligase (ligA) from Mycobacterium bovis (strain BCG / Pasteur 1173P2)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to mtc:MT3094)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (691 amino acids)

>Rv3014c DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]) (Mycobacterium tuberculosis H37Rv)
VSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPE
LRTPDSPTQLVGGAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLC
ELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTIADVPERLTPGDDYPVPEVLE
VRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVK
VDEVALQRRLGSTSRAPRWAIAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAG
STVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGPVVELRDGSEREFIMPTTCPE
CGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPT
AARALATEFGSLDAIAAASTDQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRM
VDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGGKAAGSVSKKTNYVVAGDSPG
SKYDKAVELGVPILDEDGFRRLLADGPASRT