Protein Info for Rv2995c in Mycobacterium tuberculosis H37Rv

Annotation: Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00180: Iso_dh
  • amino acids 2 to 330 (329 residues), 296.5 bits, E=1.6e-92

Best Hits

Swiss-Prot: 100% identical to LEU3_MYCTA: 3-isopropylmalate dehydrogenase (leuB) from Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 100% identity to mbo:Mb3019c)

MetaCyc: 45% identical to L-tartrate dehydrogenase/D-malate dehydrogenase (decarboxylating)/meso-tartrate decarboxylase monomer (Cereibacter sphaeroides)
4.1.1.M27 [EC: 4.1.1.M27]; Tartrate dehydrogenase. [EC: 4.1.1.M27, 1.1.1.93, 4.1.1.73]; D-malate dehydrogenase (decarboxylating). [EC: 4.1.1.M27, 1.1.1.93, 4.1.1.73, 1.1.1.83]

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• D-malate degradation (1/1 steps found)
• butanol and isobutanol biosynthesis (engineered) (4/8 steps found)
• gentisate degradation II (1/4 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• superpathway of vanillin and vanillate degradation (3/10 steps found)
• syringate degradation (3/12 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• Glyoxylate and dicarboxylate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.83 or 1.1.1.85 or 1.1.1.93 or 4.1.1.73 or 4.1.1.M27

Protein Sequence (336 amino acids)

>Rv2995c Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH) (Mycobacterium tuberculosis H37Rv)
MKLAIIAGDGIGPEVTAEAVKVLDAVVPGVQKTSYDLGARRFHATGEVLPDSVVAELRNH
DAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGIDFVVV
REGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRRRKHLTLVHKTN
VLTFAGGLWLRTVDEVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGDIITDL
AAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLSHLGE
HDAAARVDRAVEAHLATRGSERLATSDVGERIAAAL