Protein Info for Rv2967c in Mycobacterium tuberculosis H37Rv

Annotation: Probable pyruvate carboxylase Pca (pyruvic carboxylase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1127 PF00289: Biotin_carb_N" amino acids 2 to 111 (110 residues), 133.2 bits, see alignment E=2.3e-42 TIGR01235: pyruvate carboxylase" amino acids 4 to 1126 (1123 residues), 1394.8 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 117 to 323 (207 residues), 222.5 bits, see alignment E=1.8e-69 PF07478: Dala_Dala_lig_C" amino acids 144 to 291 (148 residues), 26.7 bits, see alignment E=1.5e-09 PF02785: Biotin_carb_C" amino acids 339 to 446 (108 residues), 119.9 bits, see alignment E=2.3e-38 PF00682: HMGL-like" amino acids 525 to 796 (272 residues), 90.9 bits, see alignment E=4.2e-29 PF02436: PYC_OADA" amino acids 820 to 1010 (191 residues), 200.5 bits, see alignment E=1.1e-62 PF00364: Biotin_lipoyl" amino acids 1068 to 1125 (58 residues), 52.7 bits, see alignment 1.2e-17

Best Hits

KEGG orthology group: K01958, pyruvate carboxylase [EC: 6.4.1.1] (inferred from 78% identity to gbr:Gbro_1816)

MetaCyc: 83% identical to pyruvate carboxylase subunit (Mycolicibacterium smegmatis)
Pyruvate carboxylase. [EC: 6.4.1.1]

Predicted SEED Role

"Pyruvate carboxylase (EC 6.4.1.1)" (EC 6.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1127 amino acids)

>Rv2967c Probable pyruvate carboxylase Pca (pyruvic carboxylase) (Mycobacterium tuberculosis H37Rv)
VFSKVLVANRGEIAIRAFRAAYELGVGTVAVYPYEDRNSQHRLKADESYQIGDIGHPVHA
YLSVDEIVATARRAGADAIYPGYGFLSENPDLAAACAAAGISFVGPSAEVLELAGNKSRA
IAAAREAGLPVLMSSAPSASVDELLSVAAGMPFPLFVKAVAGGGGRGMRRVGDIAALPEA
IEAASREAESAFGDPTVYLEQAVINPRHIEVQILADNLGDVIHLYERDCSVQRRHQKVIE
LAPAPHLDAELRYKMCVDAVAFARHIGYSCAGTVEFLLDERGEYVFIEMNPRVQVEHTVT
EEITDVDLVASQLRIAAGETLEQLGLRQEDIAPHGAALQCRITTEDPANGFRPDTGRISA
LRTAGGAGVRLDGSTNLGAEISPYFDSMLVKLTCRGRDLPTAVSRARRAIAEFRIRGVST
NIPFLQAVLDDPDFRAGRVTTSFIDERPQLLTARASADRGTKILNFLADVTVNNPYGSRP
STIYPDDKLPDLDLRAAPPAGSKQRLVKLGPEGFARWLRESAAVGVTDTTFRDAHQSLLA
TRVRTSGLSRVAPYLARTMPQLLSVECWGGATYDVALRFLKEDPWERLATLRAAMPNICL
QMLLRGRNTVGYTPYPEIVTSAFVQEATATGIDIFRIFDALNNIESMRPAIDAVRETGSA
IAEVAMCYTGDLTDPGEQLYTLDYYLKLAEQIVDAGAHVLAIKDMAGLLRPPAAQRLVSA
LRSRFDLPVHLHTHDTPGGQLASYVAAWHAGADAVDGAAAPLAGTTSQPALSSIVAAAAH
TEYDTGLSLSAVCALEPYWEALRKVYAPFESGLPGPTGRVYHHEIPGGQLSNLRQQAIAL
GLGDRFEEIEEAYAGADRVLGRLVKVTPTSKVVGDLALALVGAGVSADEFASDPARFGIP
ESVLGFLRGELGDPPGGWPEPLRTAALAGRGAARPTAQLAADDEIALSSVGAKRQATLNR
LLFPSPTKEFNEHREAYGDTSQLSANQFFYGLRQGEEHRVKLERGVELLIGLEAISEPDE
RGMRTVMCILNGQLRPVLVRDRSIASAVPAAEKADRGNPGHIAAPFAGVVTVGVCVGERV
GAGQTIATIEAMKMEAPITAPVAGTVERVAVSDTAQVEGGDLLVVVS