Protein Info for Rv2965c in Mycobacterium tuberculosis H37Rv

Annotation: Probable phosphopantetheine adenylyltransferase KdtB (pantetheine-phosphate adenylyltransferase) (PPAT) (dephospho-CoA pyrophosphorylase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 161 TIGR01510: pantetheine-phosphate adenylyltransferase" amino acids 4 to 155 (152 residues), 201.2 bits, see alignment E=9.5e-64 TIGR00125: cytidyltransferase-like domain" amino acids 4 to 63 (60 residues), 62.4 bits, see alignment E=3.6e-21 PF01467: CTP_transf_like" amino acids 5 to 132 (128 residues), 87.8 bits, see alignment E=7.5e-29 PF08218: Citrate_ly_lig" amino acids 11 to 74 (64 residues), 29 bits, see alignment E=8.5e-11

Best Hits

Swiss-Prot: 100% identical to COAD_MYCTU: Phosphopantetheine adenylyltransferase (coaD) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K00954, pantetheine-phosphate adenylyltransferase [EC: 2.7.7.3] (inferred from 100% identity to mtc:MT3043)

MetaCyc: 44% identical to pantetheine-phosphate adenylyltransferase (Escherichia coli K-12 substr. MG1655)
Pantetheine-phosphate adenylyltransferase. [EC: 2.7.7.3]

Predicted SEED Role

"Phosphopantetheine adenylyltransferase (EC 2.7.7.3)" in subsystem Coenzyme A Biosynthesis (EC 2.7.7.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (161 amino acids)

>Rv2965c Probable phosphopantetheine adenylyltransferase KdtB (pantetheine-phosphate adenylyltransferase) (PPAT) (dephospho-CoA pyrophosphorylase) (Mycobacterium tuberculosis H37Rv)
MTGAVCPGSFDPVTLGHVDIFERAAAQFDEVVVAILVNPAKTGMFDLDERIAMVKESTTH
LPNLRVQVGHGLVVDFVRSCGMTAIVKGLRTGTDFEYELQMAQMNKHIAGVDTFFVATAP
RYSFVSSSLAKEVAMLGGDVSELLPEPVNRRLRDRLNTERT