Protein Info for Rv2931 in Mycobacterium tuberculosis H37Rv

Annotation: Phenolpthiocerol synthesis type-I polyketide synthase PpsA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00550: PP-binding
  • amino acids 14 to 79 (66 residues), 48.3 bits, see alignment (E = 3.5e-16)
  • amino acids 1767 to 1832 (66 residues), 61.6 bits, see alignment (E = 2.5e-20)
• PF00109: ketoacyl-synt
  • amino acids 102 to 351 (250 residues), 328.5 bits, E=1.1e-101
• PF02801: Ketoacyl-synt_C
  • amino acids 359 to 477 (119 residues), 153.4 bits, 9.1e-49
• PF16197: KAsynt_C_assoc
  • amino acids 480 to 584 (105 residues), 59 bits, 2e-19
• PF00698: Acyl_transf_1
  • amino acids 632 to 950 (319 residues), 418.1 bits, E=1e-128
• PF21089: PKS_DH_N
  • amino acids 994 to 1079 (86 residues), 33.3 bits, 1.4e-11
• PF08659: KR
  • amino acids 1491 to 1683 (193 residues), 202.4 bits, E=2e-63

Best Hits

Swiss-Prot: 100% identical to PPSA_MYCTU: Phthiocerol synthesis polyketide synthase type I PpsA (ppsA) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K12440, phenolpthiocerol synthesis type-I polyketide synthase A (inferred from 100% identity to mbo:Mb2956)

MetaCyc: 100% identical to phthiodiolenone synthase (Mycobacterium tuberculosis variant bovis)
RXN-17215 [EC: 2.3.1.292]; 2.3.1.292 [EC: 2.3.1.292]; 2.3.1.292 [EC: 2.3.1.292]; 2.3.1.292 [EC: 2.3.1.292]; 2.3.1.292 [EC: 2.3.1.292]

Predicted SEED Role

"Phenolpthiocerol synthesis type-I polyketide synthase PpsA (EC 2.3.1.41)" (EC 2.3.1.41)

MetaCyc Pathways

• mycolate biosynthesis (165/205 steps found)
• superpathway of fatty acid biosynthesis II (plant) (34/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (24/31 steps found)
• superpathway of fatty acid biosynthesis initiation (5/5 steps found)
• biotin biosynthesis I (12/15 steps found)
• superpathway of fatty acids biosynthesis (E. coli) (39/53 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• fatty acid biosynthesis initiation (mitochondria) (3/4 steps found)
• gondoate biosynthesis (anaerobic) (3/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (8/11 steps found)
• stearate biosynthesis IV (4/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (6/9 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (11/16 steps found)
• fatty acid elongation -- saturated (3/5 steps found)
• phthiocerol biosynthesis (5/8 steps found)
• oleate biosynthesis IV (anaerobic) (9/14 steps found)
• (5Z)-dodecenoate biosynthesis I (3/6 steps found)
• (5Z)-dodecenoate biosynthesis II (3/6 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (12/20 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (6/12 steps found)
• palmitate biosynthesis III (16/29 steps found)
• superpathway of mycolate biosynthesis (166/239 steps found)
• tetradecanoate biosynthesis (mitochondria) (12/25 steps found)
• streptorubin B biosynthesis (15/34 steps found)
• phenolphthiocerol biosynthesis (7/23 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.41

Use Curated BLAST to search for 2.3.1.292 or 2.3.1.41

Protein Sequence (1876 amino acids)

>Rv2931 Phenolpthiocerol synthesis type-I polyketide synthase PpsA (Mycobacterium tuberculosis H37Rv)
MTGSISGEADLRHWLIDYLVTNIGCTPDEVDPDLSLADLGVSSRDAVVLSGELSELLGRT
VSPIDFWEHPTINALAAYLAAPEPSPDSDAAVKRGARNSLDEPIAVVGMGCRFPGGISCP
EALWDFLCERRSSISQVPPQRWQPFEGGPPEVAAALARTTRWGSFLPDIDAFDAEFFEIS
PSEADKMDPQQRLLLEVAWEALEHAGIPPGTLRRSATGVFAGACLSEYGAMASADLSQVD
GWSNSGGAMSIIANRLSYFLDLRGPSVAVDTACSSSLVAIHLACQSLRTQDCHLAIAAGV
NLLLSPAVFRGFDQVGALSPTGQCRAFDATADGFVRGEGAGVVVLKRLTDAQRDGDRVLA
VICGSAVNQDGRSNGLMAPNPAAQMAVLRAAYTNAGMQPSEVDYVEAHGTGTLLGDPIEA
RALGTVLGRGRPEDSPLLIGSVKTNLGHTEAAAGIAGFIKTVLAVQHGQIPPNQHFETAN
PHIPFTDLRMKVVDTQTEWPATGHPRRAGVSSFGFGGTNAHVVIEQGQEVRPAPGQGLSP
AVSTLVVAGKTMQRVSATAGMLADWMEGPGADVALADVAHTLNHHRSRQPKFGTVVARDR
TQAIAGLRALAAGQHAPGVVNPADGSPGPGTVFVYSGRGSQWAGMGRQLLADEPAFAAAV
AELEPVFVEQAGFSLHDVLANGEELVGIEQIQLGLIGMQLALTELWCSYGVRPDLVIGHS
MGEVAAAVVAGALTPAEGLRVTATRSRLMAPLSGQGGMALLELDAPTTEALIADFPQVTL
GIYNSPRQTVIAGPTEQIDELIARVRAQNRFASRVNIEVAPHNPAMDALQPAMRSELADL
TPRTPTIGIISTTYADLHTQPVFDAEHWATNMRNPVRFQQAIASAGSGADGAYHTFIEIS
AHPLLTQAIIDTLHSAQPGARYTSLGTLQRDTDDVVTFRTNLNKAHTIHPPHTPHPPEPH
PPIPTTPWQHTRHWITTKYPAGSVGSAPRAGTLLGQHTTVATVSASPPSHLWQARLAPDA
KPYQGGHRFHQVEVVPASVVLHTILSAATELGYSALSEVRFEQPIFADRPRLIQVVADNR
AISLASSPAAGTPSDRWTRHVTAQLSSSPSDSASSLNEHHRANGQPPERAHRDLIPDLAE
LLAMRGIDGLPFSWTVASWTQHSSNLTVAIDLPEALPEGSTGPLLDAAVHLAALSDVADS
RLYVPASIEQISLGDVVTGPRSSVTLNRTAHDDDGITVDVTVAAHGEVPSLSMRSLRYRA
LDFGLDVGRAQPPASTGPVEAYCDATNFVHTIDWQPQTVPDATHPGAEQVTHPGPVAIIG
DDGAALCETLEGAGYQPAVMSDGVSQARYVVYVADSDPAGADETDVDFAVRICTEITGLV
RTLAERDADKPAALWILTRGVHESVAPSALRQSFLWGLAGVIAAEHPELWGGLVDLAIND
DLGEFGPALAELLAKPSKSILVRRDGVVLAPALAPVRGEPARKSLQCRPDAAYLITGGLG
ALGLLMADWLADRGAHRLVLTGRTPLPPRRDWQLDTLDTELRRRIDAIRALEMRGVTVEA
VAADVGCREDVQALLAARDRDGAAPIRGIIHAAGITNDQLVTSMTGDAVRQVMWPKIGGS
QVLHDAFPPGSVDFFYLTASAAGIFGIPGQGSYAAANSYLDALARARRQQGCHTMSLDWV
AWRGLGLAADAQLVSEELARMGSRDITPSEAFTAWEFVDGYDVAQAVVVPMPAPAGADGS
GANAYLLPARNWSVMAATEVRSELEQGLRRIIAAELRVPEKELDTDRPFAELGLNSLMAM
AIRREAEQFVGIELSATMLFNHPTVKSLASYLAKRVAPHDVSQDNQISALSSSAGSVLDS
LFDRIESAPPEAERSV