Protein Info for Rv2502c in Mycobacterium tuberculosis H37Rv

Annotation: Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01039: Carboxyl_trans
  • amino acids 42 to 519 (478 residues), 522.9 bits, E=8e-161

Best Hits

KEGG orthology group: K01969, 3-methylcrotonyl-CoA carboxylase beta subunit [EC: 6.4.1.4] (inferred from 100% identity to mra:MRA_2528)

MetaCyc: 65% identical to propionyl-CoA carboxylase alpha subunit (Chloroflexus aurantiacus)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

• mycolate biosynthesis (165/205 steps found)
• propanoyl CoA degradation I (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• L-leucine degradation I (5/6 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• superpathway of L-methionine salvage and degradation (10/16 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of mycolate biosynthesis (166/239 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3, 6.4.1.4

Use Curated BLAST to search for 6.4.1.3 or 6.4.1.4

Protein Sequence (529 amino acids)

>Rv2502c Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD1 (Mycobacterium tuberculosis H37Rv)
VTTPSIAIAPSFADEHRRLVAELNNKLAAAALGGNERARKRHVSRGKLLPRERVDRLLDP
GSPFLELAPLAAGGMYGDESPGAGIITGIGRVSGRQCVIVANDATVKGGTYYPMTVKKHL
RAQEVALQNMLPCIYLVDSGGAFLPRQDEVFPDREHFGRIFYNQATMSAKGIPQVAAVLG
SCTAGGAYVPAMSDEAVIVREQGTIFLGGPPLVKAATGEIVSAEELGGGDLHSRTSGVTD
HLADDDEDALRIVRAIADTFGPCEPAQWDVRRSVEPKYPQAELYDVVPPDPRVPYDVHEV
VVRIVDGSEFSEFKAKYGKTLVTAFARVHGHPVGIVANNGVLFSESALKGAHFIELCDKR
KIPLLFLQNIAGFMVGRDYEAGGIAKHGAKMVTAVACARVPKLTVVIGGSYGAGNYSMCG
RAYSPRFLWMWPNARISVMGGEQAASVLATVRGEQLSAAGTPWSPDEEEAFKAPIRAQYE
DQGNPYYSTARLWDDGIIDPADTRTVVGLALSLCAHAPLDQVGYGVFRM