Protein Info for Rv2500c in Mycobacterium tuberculosis H37Rv

Annotation: Possible acyl-CoA dehydrogenase FadE19 (MMGC)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 15 to 125 (111 residues), 133 bits, E=1.2e-42
• PF02770: Acyl-CoA_dh_M
  • amino acids 130 to 230 (101 residues), 76.6 bits, E=2.7e-25
• PF00441: Acyl-CoA_dh_1
  • amino acids 243 to 391 (149 residues), 166.1 bits, E=1.2e-52
• PF08028: Acyl-CoA_dh_2
  • amino acids 259 to 374 (116 residues), 68.3 bits, E=1.6e-22

Best Hits

Swiss-Prot: 50% identical to ACDC_BACSU: Probable acyl-CoA dehydrogenase YngJ (yngJ) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to mbt:JTY_2514)

MetaCyc: 68% identical to acyl-CoA dehydrogenase subunit (Streptomyces avermitilis)
2-METHYLACYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.5]; 1.3.8.5 [EC: 1.3.8.5]; RXN0-2301 [EC: 1.3.8.5, 1.3.8.4]

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

• L-valine degradation I (7/8 steps found)
• L-isoleucine degradation I (5/6 steps found)
• L-leucine degradation I (5/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• valproate β-oxidation (6/9 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4

Use Curated BLAST to search for 1.3.8.4 or 1.3.8.5

Protein Sequence (394 amino acids)

>Rv2500c Possible acyl-CoA dehydrogenase FadE19 (MMGC) (Mycobacterium tuberculosis H37Rv)
MTTTTTTISGGILPKEYQDLRDTVADFARTVVAPVSAKHDAEHSFPYEIVAKMGEMGLFG
LPFPEEYGGMGGDYFALSLVLEELGKVDQSVAITLEAAVGLGAMPIYRFGTEEQKQKWLP
DLTSGRALAGFGLTEPGAGSDAGSTRTTARLEGDEWIINGSKQFITNSGTDITSLVTVTA
VTGTTGTAADAKKEISTIIVPSGTPGFTVEPVYNKVGWNASDTHPLTFADARVPRENLLG
ARGSGYANFLSILDEGRIAIAALATGAAQGCVDESVKYANQRQSFGQPIGAYQAIGFKIA
RMEARAHVARTAYYDAAAKMLAGKPFKKEAAIAKMISSEAAMDNSRDATQIHGGYGFMNE
YPVARHYRDSKVLEIGEGTTEVQLMLIARSLGLQ