Protein Info for Rv2448c in Mycobacterium tuberculosis H37Rv

Annotation: Probable valyl-tRNA synthase protein ValS (valyl-tRNA synthetase) (valine--tRNA ligase) (valine translase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876 transmembrane" amino acids 482 to 486 (5 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 3 to 854 (852 residues), 992.9 bits, see alignment E=6.5e-303 PF00133: tRNA-synt_1" amino acids 15 to 426 (412 residues), 312.2 bits, see alignment E=1.3e-96 amino acids 436 to 569 (134 residues), 152.8 bits, see alignment E=3.3e-48 PF09334: tRNA-synt_1g" amino acids 43 to 90 (48 residues), 25.3 bits, see alignment 1.7e-09 amino acids 517 to 579 (63 residues), 27.9 bits, see alignment 2.6e-10 PF13603: tRNA-synt_1_2" amino acids 242 to 300 (59 residues), 25.6 bits, see alignment 2.1e-09 PF08264: Anticodon_1" amino acids 611 to 750 (140 residues), 124.2 bits, see alignment E=1e-39 PF10458: Val_tRNA-synt_C" amino acids 811 to 875 (65 residues), 60.8 bits, see alignment 3.1e-20

Best Hits

Swiss-Prot: 100% identical to SYV_MYCTO: Valine--tRNA ligase (valS) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 100% identity to mtu:Rv2448c)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (876 amino acids)

>Rv2448c Probable valyl-tRNA synthase protein ValS (valyl-tRNA synthetase) (valine--tRNA ligase) (valine translase) (Mycobacterium tuberculosis H37Rv)
MLPKSWDPAAMESAIYQKWLDAGYFTADPTSTKPAYSIVLPPPNVTGSLHMGHALEHTMM
DALTRRKRMQGYEVLWQPGTDHAGIATQSVVEQQLAVDGKTKEDLGRELFVDKVWDWKRE
SGGAIGGQMRRLGDGVDWSRDRFTMDEGLSRAVRTIFKRLYDAGLIYRAERLVNWSPVLQ
TAISDLEVNYRDVEGELVSFRYGSLDDSQPHIVVATTRVETMLGDTAIAVHPDDERYRHL
VGTSLAHPFVDRELAIVADEHVDPEFGTGAVKVTPAHDPNDFEIGVRHQLPMPSILDTKG
RIVDTGTRFDGMDRFEARVAVRQALAAQGRVVEEKRPYLHSVGHSERSGEPIEPRLSLQW
WVRVESLAKAAGDAVRNGDTVIHPASMEPRWFSWVDDMHDWCISRQLWWGHRIPIWYGPD
GEQVCVGPDETPPQGWEQDPDVLDTWFSSALWPFSTLGWPDKTAELEKFYPTSVLVTGYD
ILFFWVARMMMFGTFVGDDAAITLDGRRGPQVPFTDVFLHGLIRDESGRKMSKSKGNVID
PLDWVEMFGADALRFTLARGASPGGDLAVSEDAVRASRNFGTKLFNATRYALLNGAAPAP
LPSPNELTDADRWILGRLEEVRAEVDSAFDGYEFSRACESLYHFAWDEFCDWYLELAKTQ
LAQGLTHTTAVLAAGLDTLLRLLHPVIPFLTEALWLALTGRESLVSADWPEPSGISVDLV
AAQRINDMQKLVTEVRRFRSDQGLADRQKVPARMHGVRDSDLSNQVAAVTSLAWLTEPGP
DFEPSVSLEVRLGPEMNRTVVVELDTSGTIDVAAERRRLEKELAGAQKELASTAAKLANA
DFLAKAPDAVIAKIRDRQRVAQQETERITTRLAALQ