Protein Info for Rv2301 in Mycobacterium tuberculosis H37Rv

Annotation: Probable cutinase Cut2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 230 signal peptide" amino acids 1 to 44 (44 residues), see Phobius details PF01083: Cutinase" amino acids 44 to 229 (186 residues), 164.9 bits, see alignment E=9.2e-53

Best Hits

Swiss-Prot: 100% identical to CUT2_MYCTO: Probable cutinase cut2 (cut2) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K08095, cutinase [EC: 3.1.1.74] (inferred from 100% identity to mra:MRA_2318)

Predicted SEED Role

"serine esterase, cutinase family"

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.74

Use Curated BLAST to search for 3.1.1.74

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (230 amino acids)

>Rv2301 Probable cutinase Cut2 (Mycobacterium tuberculosis H37Rv)
VNDLLTRRLLTMGAAAAMLAAVLLLTPITVPAGYPGAVAPATAACPDAEVVFARGRFEPP
GIGTVGNAFVSALRSKVNKNVGVYAVKYPADNQIDVGANDMSAHIQSMANSCPNTRLVPG
GYSLGAAVTDVVLAVPTQMWGFTNPLPPGSDEHIAAVALFGNGSQWVGPITNFSPAYNDR
TIELCHGDDPVCHPADPNTWEANWPQHLAGAYVSSGMVNQAADFVAGKLQ