Protein Info for Rv2130c in Mycobacterium tuberculosis H37Rv

Annotation: Cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase MshC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 TIGR03447: cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase" amino acids 2 to 414 (413 residues), 685.2 bits, see alignment E=1.5e-210 PF01406: tRNA-synt_1e" amino acids 36 to 338 (303 residues), 279.5 bits, see alignment E=3.7e-87

Best Hits

Swiss-Prot: 100% identical to MSHC_MYCBP: L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) from Mycobacterium bovis (strain BCG / Pasteur 1173P2)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 100% identity to mbb:BCG_2147c)

MetaCyc: 79% identical to L-cysteine : 1 D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase small subunit (Mycolicibacterium smegmatis)
RXN1G-121 [EC: 6.3.1.13]

Predicted SEED Role

"L-cysteine:1D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase MshC" in subsystem Glutathione analogs: mycothiol

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 6.1.1.16 or 6.3.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (414 amino acids)

>Rv2130c Cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase MshC (Mycobacterium tuberculosis H37Rv)
MQSWYCPPVPVLPGRGPQLRLYDSADRQVRPVAPGSKATMYVCGITPYDATHLGHAATYV
TFDLIHRLWLDLGHELHYVQNITDIDDPLFERADRDGVDWRDLAQAEVALFCEDMAALRV
LPPQDYVGATEAIAEMVELIEKMLACGAAYVIDREMGEYQDIYFRADATLQFGYESGYDR
DTMLRLCEERGGDPRRPGKSDELDALLWRAARPGEPSWPSPFGPGRPGWHVECAAIALSR
IGSGLDIQGGGSDLIFPHHEFTAAHAECVSGERRFARHYVHAGMIGWDGHKMSKSRGNLV
LVSALRAQDVEPSAVRLGLLAGHYRADRFWSQQVLDEATARLHRWRTATALPAGPAAVDV
VARVRRYLADDLDTPKAIAALDGWVTDAVEYGGHDAGAPKLVATAIDALLGVDL