Protein Info for Rv2124c in Mycobacterium tuberculosis H37Rv

Annotation: 5-methyltetrahydrofolate--homocystein methyltransferase MetH (methionine synthase, vitamin-B12 dependent isozyme) (ms)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 17 to 1192 (1176 residues), 1712.9 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 22 to 312 (291 residues), 272.6 bits, E=1.4e-84
• PF00809: Pterin_bind
  • amino acids 347 to 583 (237 residues), 164.7 bits, E=7.9e-52
• PF02607: B12-binding_2
  • amino acids 644 to 717 (74 residues), 80.4 bits, 2.2e-26
• PF02310: B12-binding
  • amino acids 730 to 849 (120 residues), 68.5 bits, E=1.2e-22
• PF02965: Met_synt_B12
  • amino acids 978 to 1192 (215 residues), 110 bits, E=3.8e-35

Best Hits

Swiss-Prot: 100% identical to METH_MYCTU: Methionine synthase (metH) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 77% identity to asd:AS9A_1963)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine salvage from L-homocysteine (2/3 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• folate transformations II (plants) (7/11 steps found)
• superpathway of L-methionine salvage and degradation (10/16 steps found)
• folate transformations I (6/13 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (1192 amino acids)

>Rv2124c 5-methyltetrahydrofolate--homocystein methyltransferase MetH (methionine synthase, vitamin-B12 dependent isozyme) (ms) (Mycobacterium tuberculosis H37Rv)
VTAADKHLYDTDLLDVLSQRVMVGDGAMGTQLQAADLTLDDFRGLEGCNEILNETRPDVL
ETIHRNYFEAGADAVETNTFGCNLSNLGDYDIADRIRDLSQKGTAIARRVADELGSPDRK
RYVLGSMGPGTKLPTLGHTEYAVIRDAYTEAALGMLDGGADAILVETCQDLLQLKAAVLG
SRRAMTRAGRHIPVFAHVTVETTGTMLLGSEIGAALTAVEPLGVDMIGLNCATGPAEMSE
HLRHLSRHARIPVSVMPNAGLPVLGAKGAEYPLLPDELAEALAGFIAEFGLSLVGGCCGT
TPAHIREVAAAVANIKRPERQVSYEPSVSSLYTAIPFAQDASVLVIGERTNANGSKGFRE
AMIAEDYQKCLDIAKDQTRDGAHLLDLCVDYVGRDGVADMKALASRLATSSTLPIMLDST
ETAVLQAGLEHLGGRCAINSVNYEDGDGPESRFAKTMALVAEHGAAVVALTIDEEGQART
AQKKVEIAERLINDITGNWGVDESSILIDTLTFTIATGQEESRRDGIETIEAIRELKKRH
PDVQTTLGLSNISFGLNPAARQVLNSVFLHECQEAGLDSAIVHASKILPMNRIPEEQRNV
ALDLVYDRRREDYDPLQELMRLFEGVSAASSKEDRLAELAGLPLFERLAQRIVDGERNGL
DADLDEAMTQKPPLQIINEHLLAGMKTVGELFGSGQMQLPFVLQSAEVMKAAVAYLEPHM
ERSDDDSGKGRIVLATVKGDVHDIGKNLVDIILSNNGYEVVNIGIKQPIATILEVAEDKS
ADVVGMSGLLVKSTVVMKENLEEMNTRGVAEKFPVLLGGAALTRSYVENDLAEIYQGEVH
YARDAFEGLKLMDTIMSAKRGEAPDENSPEAIKAREKEAERKARHQRSKRIAAQRKAAEE
PVEVPERSDVAADIEVPAPPFWGSRIVKGLAVADYTGLLDERALFLGQWGLRGQRGGEGP
SYEDLVETEGRPRLRYWLDRLSTDGILAHAAVVYGYFPAVSEGNDIVVLTEPKPDAPVRY
RFHFPRQQRGRFLCIADFIRSRELAAERGEVDVLPFQLVTMGQPIADFANELFASNAYRD
YLEVHGIGVQLTEALAEYWHRRIREELKFSGDRAMAAEDPEAKEDYFKLGYRGARFAFGY
GACPDLEDRAKMMALLEPERIGVTLSEELQLHPEQSTDAFVLHHPEAKYFNV