Protein Info for Rv1633 in Mycobacterium tuberculosis H37Rv

Annotation: Probable excinuclease ABC (subunit B-helicase) UvrB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 TIGR00631: excinuclease ABC subunit B" amino acids 6 to 686 (681 residues), 1089.2 bits, see alignment E=0 PF04851: ResIII" amino acids 18 to 89 (72 residues), 37.5 bits, see alignment E=8.1e-13 PF00270: DEAD" amino acids 19 to 92 (74 residues), 28 bits, see alignment E=5.8e-10 PF17757: UvrB_inter" amino acids 161 to 250 (90 residues), 113.2 bits, see alignment E=1.8e-36 PF27431: UvrB_3rd" amino acids 255 to 316 (62 residues), 103.4 bits, see alignment 1.7e-33 PF00271: Helicase_C" amino acids 435 to 546 (112 residues), 67.7 bits, see alignment E=3.6e-22 PF12344: UvrB" amino acids 553 to 594 (42 residues), 78.2 bits, see alignment 1.2e-25 PF02151: UVR" amino acids 655 to 687 (33 residues), 29.3 bits, see alignment (E = 1.9e-10)

Best Hits

Swiss-Prot: 100% identical to UVRB_MYCTO: UvrABC system protein B (uvrB) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K03702, excinuclease ABC subunit B (inferred from 100% identity to mbt:JTY_1646)

MetaCyc: 61% identical to UvrABC excision nuclease subunit B (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit B" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (698 amino acids)

>Rv1633 Probable excinuclease ABC (subunit B-helicase) UvrB (Mycobacterium tuberculosis H37Rv)
VRAGGHFEVVSPHAPAGDQPAAIDELERRINAGERDVVLLGATGTGKSATTAWLIERLQR
PTLVMAPNKTLAAQLANELREMLPHNAVEYFVSYYDYYQPEAYIAQTDTYIEKDSSINDD
VERLRHSATSALLSRRDVVVVASVSCIYGLGTPQSYLDRSVELKVGEEVPRDGLLRLLVD
VQYTRNDMSFTRGSFRVRGDTVEIIPSYEELAVRIEFFGDEIEALYYLHPLTGEVIRQVD
SLRIFPATHYVAGPERMAHAVSAIEEELAERLAELESQGKLLEAQRLRMRTNYDIEMMRQ
VGFCSGIENYSRHIDGRGPGTPPATLLDYFPEDFLLVIDESHVTVPQIGGMYEGDISRKR
NLVEYGFRLPSACDNRPLTWEEFADRIGQTVYLSATPGPYELSQTGGEFVEQVIRPTGLV
DPKVVVKPTKGQIDDLIGEIRTRADADQRVLVTTLTKKMAEDLTDYLLEMGIRVRYLHSE
VDTLRRVELLRQLRLGDYDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSSRSLIQTI
GRAARNVSGEVHMYADKITDSMREAIDETERRRAKQIAYNEANGIDPQPLRKKIADILDQ
VYREADDTAVVEVGGSGRNASRGRRAQGEPGRAVSAGVFEGRDTSAMPRAELADLIKDLT
AQMMAAARDLQFELAARFRDEIADLKRELRGMDAAGLK