Protein Info for Rv1328 in Mycobacterium tuberculosis H37Rv

Annotation: Probable glycogen phosphorylase GlgP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 PF11897: DUF3417" amino acids 13 to 126 (114 residues), 118.4 bits, see alignment E=2e-38 TIGR02094: alpha-glucan phosphorylases" amino acids 113 to 721 (609 residues), 779.6 bits, see alignment E=1.2e-238 PF00343: Phosphorylase" amino acids 235 to 632 (398 residues), 75.7 bits, see alignment E=2.6e-25

Best Hits

Swiss-Prot: 100% identical to PHSG_MYCTU: Glycogen phosphorylase (glgP) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to mtf:TBFG_11360)

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (863 amino acids)

>Rv1328 Probable glycogen phosphorylase GlgP (Mycobacterium tuberculosis H37Rv)
VKALRRFTVRAHLPERLAALDQLSTNLRWSWDKPTQDLFAAIDPALWEQCGHDPVALLGA
VNPARLDELALDAEFLGALDELAADLNDYLSRPLWYQEQQDAGVAAQALPTGIAYFSLEF
GVAEVLPNYSGGLGILAGDHLKSASDLGVPLIAVGLYYRSGYFRQSLTADGWQHETYPSL
DPQGLPLRLLTDANGDPVLVEVALGDNAVLRARIWVAQVGRVPLLLLDSDIPENEHDLRN
VTDRLYGGDQEHRIKQEILAGIGGVRAIRAYTAVEKLTPPEVFHMNEGHAGFLGIERIRE
LVTDAGLDFDTALTVVRSSTVFTTHTPVPAGIDRFPLEMVQRYVNDQRGDGRSRLLPGLP
ADRIVALGAEDDPAKFNMAHMGLRLAQRANGVSLLHGRVSRAMFNELWAGFDPDEVPIGS
VTNGVHAPTWAAPQWLQLGRELAGSDSLREPVVWQRLHQVDPAHLWWIRSQLRSMLVEDV
RARLRQSWLERGATDAELGWIATAFDPNVLTVGFARRVPTYKRLTLMLRDPDRLEQLLLD
EQRPIQLIVAGKSHPADDGGKALIQQVVRFADRPQVRHRIAFLPNYDMSMARLLYWGCDV
WLNNPLRPLEACGTSGMKSALNGGLNLSIRDGWWDEWYDGENGWEIPSADGVADENRRDD
LEAGALYDLLAQAVAPKFYERDERGVPQRWVEMVRHTLQTLGPKVLASRMVRDYVEHYYA
PAAQSFRRTAGAQFDAARELADYRRRAEEAWPKIEIADVDSTGLPDTPLLGSQLTLTATV
RLAGLRPNDVTVQGVLGRVDAGDVLMDPVTVEMAHTGTGDGGYEIFSTTTPLPLAGPVGY
TVRVLPRHPMLAASNELGLVTLA