Protein Info for Rv1317c in Mycobacterium tuberculosis H37Rv

Annotation: Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF02805: Ada_Zn_binding" amino acids 8 to 71 (64 residues), 98.1 bits, see alignment E=5.8e-32 PF12833: HTH_18" amino acids 106 to 185 (80 residues), 56.5 bits, see alignment E=7e-19 PF06029: AlkA_N" amino acids 202 to 316 (115 residues), 122.5 bits, see alignment E=2.9e-39 PF00730: HhH-GPD" amino acids 322 to 470 (149 residues), 26.9 bits, see alignment E=1.3e-09

Best Hits

Swiss-Prot: 100% identical to ALKA_MYCTO: Probable bifunctional transcriptional activator/DNA repair enzyme AlkA (alkA) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K13529, AraC family transcriptional regulator, regulatory protein of adaptative response / DNA-3-methyladenine glycosylase II [EC: 3.2.2.21] (inferred from 100% identity to mbt:JTY_1352)

Predicted SEED Role

"Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63, 3.2.2.21

Use Curated BLAST to search for 2.1.1.63 or 3.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (496 amino acids)

>Rv1317c Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase) (Mycobacterium tuberculosis H37Rv)
VHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGE
GFRACKRCRPDASPGSPEWNVRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERL
LQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAGFSSIRQFNDTVRLACDGTPT
ALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLR
AVVGKAPGQRIPRTVDEAEFAVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHT
FPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLDAGCDWQRARGQLLALPGVGP
WTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
TLEHPVNQWPPQEKIA