Protein Info for Rv1257c in Mycobacterium tuberculosis H37Rv

Annotation: Probable oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01565: FAD_binding_4
  • amino acids 41 to 179 (139 residues), 147.3 bits, E=2.5e-47
• PF02913: FAD-oxidase_C
  • amino acids 218 to 452 (235 residues), 233.3 bits, E=3.8e-73

Best Hits

Swiss-Prot: 41% identical to GLCD_BACSU: Glycolate oxidase subunit GlcD (glcD) from Bacillus subtilis (strain 168)

KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 100% identity to mtf:TBFG_11283)

MetaCyc: 38% identical to quinone-dependent D-lactate dehydrogenase catalytic subunit (Desulfovibrio vulgaris)
D-lactate dehydrogenase (cytochrome c-553). [EC: 1.1.2.5]

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

• glycolate and glyoxylate degradation II (2/2 steps found)
• glycolate and glyoxylate degradation III (1/3 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• glycolate and glyoxylate degradation I (1/4 steps found)
• superpathway of glycol metabolism and degradation (3/7 steps found)
• photorespiration I (4/9 steps found)
• photorespiration III (4/9 steps found)
• photorespiration II (4/10 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.5 or 1.1.3.15 or 1.1.99.14

Protein Sequence (455 amino acids)

>Rv1257c Probable oxidoreductase (Mycobacterium tuberculosis H37Rv)
VNTDVLAGLMAELPEGMVVTDPAVTDGYRQDRAFDPSAGKPLAIIRPRRTEEVQTVLRWA
SANQVPVVTRGAGSGLSGGATALDGGIVLSTEKMRDITVDPVTRTAVCQPGLYNAEVKEA
AAEHGLWYPPDPSSFEICSIGGNIATNAGGLCCVKYGVTGDYVLGMQVVLANGTAVRLGG
PRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPAQNASSIVVASFGSVQAAVDAVLGVT
GRLRPAMLEFMDSVAINAVEDTLRMDLDRDAAAMLVAGSDERGRAATEDAAVMAAVFAEN
GAIDVFSTDDPDEGEAFIAARRFAIPAVESKGALLLEDVGVPLPALGELVTGIARIAEER
NLMISVIAHAGDGNTHPLLVYDPADAAMLERAHLAYGEIMDLAVGLGGTITGEHGVGRLK
RPWLAGYLGPDVLALNQRIKQALDPQGILNPGSAI