Protein Info for Rv1248c in Mycobacterium tuberculosis H37Rv

Annotation: Multifunctional alpha-ketoglutarate metabolic enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1231 PF16078: 2-oxogl_dehyd_N" amino acids 8 to 37 (30 residues), 41.5 bits, see alignment (E = 2e-14) PF00198: 2-oxoacid_dh" amino acids 103 to 335 (233 residues), 167.5 bits, see alignment E=8.8e-53 TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 358 to 1229 (872 residues), 987.8 bits, see alignment E=2.5e-301 PF00676: E1_dh" amino acids 489 to 817 (329 residues), 177.6 bits, see alignment E=7.3e-56 PF02779: Transket_pyr" amino acids 879 to 1078 (200 residues), 139.2 bits, see alignment E=2.8e-44 PF16870: OxoGdeHyase_C" amino acids 1083 to 1230 (148 residues), 136.1 bits, see alignment E=2e-43

Best Hits

Swiss-Prot: 100% identical to KGD_MYCTU: Multifunctional 2-oxoglutarate metabolism enzyme (kgd) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K01616, 2-oxoglutarate decarboxylase [EC: 4.1.1.71] (inferred from 74% identity to asd:AS9A_3326)

MetaCyc: 100% identical to 2-hydroxy-3-oxoadipate synthase (Mycobacterium tuberculosis H37Rv)
2-hydroxy-3-oxoadipate synthase. [EC: 2.2.1.5]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.5 or 4.1.1.71

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1231 amino acids)

>Rv1248c Multifunctional alpha-ketoglutarate metabolic enzyme (Mycobacterium tuberculosis H37Rv)
VANISSPFGQNEWLVEEMYRKFRDDPSSVDPSWHEFLVDYSPEPTSQPAAEPTRVTSPLV
AERAAAAAPQAPPKPADTAAAGNGVVAALAAKTAVPPPAEGDEVAVLRGAAAAVVKNMSA
SLEVPTATSVRAVPAKLLIDNRIVINNQLKRTRGGKISFTHLLGYALVQAVKKFPNMNRH
YTEVDGKPTAVTPAHTNLGLAIDLQGKDGKRSLVVAGIKRCETMRFAQFVTAYEDIVRRA
RDGKLTTEDFAGVTISLTNPGTIGTVHSVPRLMPGQGAIIGVGAMEYPAEFQGASEERIA
ELGIGKLITLTSTYDHRIIQGAESGDFLRTIHELLLSDGFWDEVFRELSIPYLPVRWSTD
NPDSIVDKNARVMNLIAAYRNRGHLMADTDPLRLDKARFRSHPDLEVLTHGLTLWDLDRV
FKVDGFAGAQYKKLRDVLGLLRDAYCRHIGVEYAHILDPEQKEWLEQRVETKHVKPTVAQ
QKYILSKLNAAEAFETFLQTKYVGQKRFSLEGAESVIPMMDAAIDQCAEHGLDEVVIGMP
HRGRLNVLANIVGKPYSQIFTEFEGNLNPSQAHGSGDVKYHLGATGLYLQMFGDNDIQVS
LTANPSHLEAVDPVLEGLVRAKQDLLDHGSIDSDGQRAFSVVPLMLHGDAAFAGQGVVAE
TLNLANLPGYRVGGTIHIIVNNQIGFTTAPEYSRSSEYCTDVAKMIGAPIFHVNGDDPEA
CVWVARLAVDFRQRFKKDVVIDMLCYRRRGHNEGDDPSMTNPYVYDVVDTKRGARKSYTE
ALIGRGDISMKEAEDALRDYQGQLERVFNEVRELEKHGVQPSESVESDQMIPAGLATAVD
KSLLARIGDAFLALPNGFTAHPRVQPVLEKRREMAYEGKIDWAFGELLALGSLVAEGKLV
RLSGQDSRRGTFSQRHSVLIDRHTGEEFTPLQLLATNSDGSPTGGKFLVYDSPLSEYAAV
GFEYGYTVGNPDAVVLWEAQFGDFVNGAQSIIDEFISSGEAKWGQLSNVVLLLPHGHEGQ
GPDHTSARIERFLQLWAEGSMTIAMPSTPSNYFHLLRRHALDGIQRPLIVFTPKSMLRHK
AAVSEIKDFTEIKFRSVLEEPTYEDGIGDRNKVSRILLTSGKLYYELAARKAKDNRNDLA
IVRLEQLAPLPRRRLRETLDRYENVKEFFWVQEEPANQGAWPRFGLELPELLPDKLAGIK
RISRRAMSAPSSGSSKVHAVEQQEILDEAFG