Protein Info for Rv1187 in Mycobacterium tuberculosis H37Rv

Annotation: Probable pyrroline-5-carboxylate dehydrogenase RocA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01236: 1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 11 to 540 (530 residues), 908.7 bits, E=5.7e-278
• PF00171: Aldedh
  • amino acids 53 to 522 (470 residues), 306.6 bits, E=1.3e-95

Best Hits

Swiss-Prot: 52% identical to PUT2_SCHPO: Probable delta-1-pyrroline-5-carboxylate dehydrogenase (SPBC24C6.04) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K00294, 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12] (inferred from 100% identity to mtc:MT1224)

MetaCyc: 49% identical to Delta1-pyrroline-5-carboxylate dehydrogenase, mitochondrial subunit (Homo sapiens)
RXN-14116 [EC: 1.2.1.88]; 1.2.1.- [EC: 1.2.1.88]

Predicted SEED Role

"Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization (EC 1.5.1.12)

MetaCyc Pathways

• L-arginine degradation I (arginase pathway) (2/3 steps found)
• L-proline degradation I (2/3 steps found)
• trans-4-hydroxy-L-proline degradation I (2/5 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12

Protein Sequence (543 amino acids)

>Rv1187 Probable pyrroline-5-carboxylate dehydrogenase RocA (Mycobacterium tuberculosis H37Rv)
MDAITQVPVPANEPVHDYAPKSPERTRLRTELASLADHPIDLPHVIGGRHRMGDGERIDV
VQPHRHAARLGTLTNATHADAAAAVEAAMSAKSDWAALPFDERAAVFLRAADLLAGPWRE
KIAAATMLGQSKSVYQAEIDAVCELIDFWRFNVAFARQILEQQPISGPGEWNRIDYRPLD
GFVYAITPFNFTSIAGNLPTAPALMGNTVIWKPSITQTLAAYLTMQLLEAAGLPPGVINL
VTGDGFAVSDVALADPRLAGIHFTGSTATFGHLWQWVGTNIGRYHSYPRLVGETGGKDFV
VAHASARPDVLRTALIRGAFDYQGQKCSAVSRAFIAHSVWQRMGDELLAKAAELRYGDIT
DLSNYGGALIDQRAFVKNVDAIERAKGAAAVTVAVGGEYDDSEGYFVRPTVLLSDDPTDE
SFVIEYFGPLLSVHVYPDERYEQILDVIDTGSRYALTGAVIADDRQAVLTALDRLRFAAG
NFYVNDKPTGAVVGRQPFGGARGSGTNDKAGSPLNLLRWTSARSIKETFVAATDHIYPHM
AVD