Protein Info for Rv1162 in Mycobacterium tuberculosis H37Rv

Annotation: Probable respiratory nitrate reductase (beta chain) NarH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 TIGR01660: nitrate reductase, beta subunit" amino acids 1 to 489 (489 residues), 743.6 bits, see alignment E=4.3e-228 PF13247: Fer4_11" amino acids 177 to 272 (96 residues), 125.4 bits, see alignment E=2.2e-40 PF13237: Fer4_10" amino acids 180 to 229 (50 residues), 32.4 bits, see alignment 1.8e-11 PF14711: Nitr_red_bet_C" amino acids 360 to 441 (82 residues), 100.2 bits, see alignment E=1e-32

Best Hits

KEGG orthology group: K00371, nitrate reductase 1, beta subunit [EC: 1.7.99.4] (inferred from 100% identity to mtc:MT1199)

Predicted SEED Role

"Respiratory nitrate reductase beta chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (558 amino acids)

>Rv1162 Probable respiratory nitrate reductase (beta chain) NarH (Mycobacterium tuberculosis H37Rv)
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGVGYPRTYEDQE
RWRGGWVRDKKGRLRLRDGGRIHKLLRIFANPKLPTIGDYYEPWTYDYENLTSAPAGDTF
PTAAPRSLISGNPMKVSWGSNWDDNLAGSPEIVPNDPVLKKVNQVNQEVKLKLEETFMFY
LPRICEHCLNPSCVASCPSGAMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTG
KAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRI
LLDPHDPRVIAGARAEGIADEWIEAAQRSPVYALINTYRVALPLHPEYRTMPMVWYIPPL
SPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTAGDTEVVAGVLRRLAAMRCYM
RDINLGRETQPHIPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMG
CSLSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQHAGSAATGGAGRSRVNLLNWDPNG
AAAGLFPEPQPSKDVVQR