Protein Info for Rv1143 in Mycobacterium tuberculosis H37Rv

Annotation: Probable alpha-methylacyl-CoA racemase Mcr (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 48% identical to AMACR_RAT: Alpha-methylacyl-CoA racemase (Amacr) from Rattus norvegicus

KEGG orthology group: K01796, alpha-methylacyl-CoA racemase [EC: 5.1.99.4] (inferred from 100% identity to mtu:Rv1143)

MetaCyc: 100% identical to alpha-methylacyl-CoA racemase (Mycobacterium tuberculosis H37Rv)
Alpha-methylacyl-CoA racemase. [EC: 5.1.99.4]; 5.1.99.4 [EC: 5.1.99.4]; 5.1.99.4 [EC: 5.1.99.4]; 5.1.99.4 [EC: 5.1.99.4]

Predicted SEED Role

"Alpha-methylacyl-CoA racemase (EC 5.1.99.4)" (EC 5.1.99.4)

MetaCyc Pathways

superpathway of cholesterol degradation II (cholesterol dehydrogenase) (36/47 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (14/22 steps found)
cholesterol degradation to androstenedione III (anaerobic) (10/22 steps found)
superpathway of cholesterol degradation III (oxidase) (23/49 steps found)
bile acid biosynthesis, neutral pathway (2/29 steps found)

KEGG Metabolic Maps

Bile acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.1.99.4

Use Curated BLAST to search for 5.1.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (360 amino acids)

>Rv1143 Probable alpha-methylacyl-CoA racemase Mcr (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) (Mycobacterium tuberculosis H37Rv)
MAGPLSGLRVVELAGIGPGPHAAMILGDLGADVVRIDRPSSVDGISRDAMLRNRRIVTAD
LKSDQGLELALKLIAKADVLIEGYRPGVTERLGLGPEECAKVNDRLIYARMTGWGQTGPR
SQQAGHDINYISLNGILHAIGRGDERPVPPLNLVGDFGGGSMFLLVGILAALWERQSSGK
GQVVDAAMVDGSSVLIQMMWAMRATGMWTDTRGANMLDGGAPYYDTYECADGRYVAVGAI
EPQFYAAMLAGLGLDAAELPPQNDRARWPELRALLTEAFASHDRDHWGAVFANSDACVTP
VLAFGEVHNEPHIIERNTFYEANGGWQPMPAPRFSRTASSQPRPPAATIDIEAVLTDWDG