Protein Info for Rv0971c in Mycobacterium tuberculosis H37Rv

Annotation: Probable enoyl-CoA hydratase EchA7 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16113: ECH_2
  • amino acids 18 to 206 (189 residues), 94.5 bits, E=9.7e-31
• PF00378: ECH_1
  • amino acids 18 to 261 (244 residues), 146.7 bits, E=7.9e-47

Best Hits

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to mbo:Mb0996c)

Predicted SEED Role

"Methylglutaconyl-CoA hydratase (EC 4.2.1.18)" in subsystem Benzoate transport and degradation cluster or HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.2.1.18)

MetaCyc Pathways

• oleate β-oxidation (27/35 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• L-leucine degradation I (5/6 steps found)
• adipate biosynthesis (4/5 steps found)
• adipate degradation (4/5 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• valproate β-oxidation (6/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation I (generic) (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (2/5 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• ferrichrome A biosynthesis (1/5 steps found)
• benzoyl-CoA degradation I (aerobic) (2/7 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• superpathway of phenylethylamine degradation (4/11 steps found)
• 3-phenylpropanoate degradation (3/10 steps found)
• phenylacetate degradation I (aerobic) (2/9 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (3/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (3/14 steps found)
• platensimycin biosynthesis (7/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 4.2.1.17 or 4.2.1.18

Protein Sequence (269 amino acids)

>Rv0971c Probable enoyl-CoA hydratase EchA7 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) (Mycobacterium tuberculosis H37Rv)
MDSPVDYAGPAACGGPFARLTLNSPHNRNALSSTLVSQLHQGLSAAEADPAVRLVVLGHT
GGTFCAGADLSEAGGGGGDPYRMAVARAREMTALLRAIVESPLPVVGAINGHVRAGGFGL
VGACDMVVAGPESTFALTEARIGVAPAIISLTLLPKLSPRAAARYYLTGEKFGAREAADI
GLITMAADDVDAAVAALVADVGRGSPQGLAASKALTTAAVLEGFDRDAERLTEESARLFV
SDEAREGMLAFLQKRPPRWVQPATMRAAD