Protein Info for Rv0886 in Mycobacterium tuberculosis H37Rv

Annotation: Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to FPRB_MYCTO: Probable ferredoxin/ferredoxin--NADP reductase (fprB) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K00528, ferredoxin--NADP+ reductase [EC: 1.18.1.2] (inferred from 100% identity to mbt:JTY_0908)

Predicted SEED Role

"Ferredoxin / Ferredoxin--NADP(+) reductase, actinobacterial (eukaryote-like) type (EC 1.18.1.2)" in subsystem Soluble cytochromes and functionally related electron carriers (EC 1.18.1.2)

MetaCyc Pathways

ethene biosynthesis V (engineered) (17/25 steps found)
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) (1/4 steps found)
photosynthesis light reactions (1/4 steps found)
oxygenic photosynthesis (10/17 steps found)
1-butanol autotrophic biosynthesis (engineered) (17/27 steps found)
superpathway of photosynthetic hydrogen production (1/6 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (15/26 steps found)

KEGG Metabolic Maps

Photosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.18.1.2

Use Curated BLAST to search for 1.18.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (575 amino acids)

>Rv0886 Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase) (Mycobacterium tuberculosis H37Rv)
MPHVITQSCCNDASCVFACPVNCIHPTPDEPGFATSEMLYIDPVACVDCGACVTACPVSA
IAPNTRLDFEQLPFVEINASYYPKRPAGVKLAPTSKLAPVTPAAEVRVRRQPLTVAVVGS
GPAAMYAADELLVQQGVQVNVFEKLPTPYGLVRSGVAPDHQNTKRVTRLFDRIAGHRRFR
FYLNVEIGKHLGHAELLAHHHAVLYAVGAPDDRRLTIDGMGLPGTGTATELVAWLNGHPD
FNDLPVDLSHERVVIIGNGNVALDVARVLAADPHELAATDIADHALSALRNSAVREVVVA
ARRGPAHSAFTLPELIGLTAGADVVLDPGDHQRVLDDLAIVADPLTRNKLEILSTLGDGS
APARRVGRPRIRLAYRLTPRRVLGQRRAGGVQFSVTGTDELRQLDAGLVLTSIGYRGKPI
PDLPFDEQAALVPNDGGRVIDPGTGEPVPGAYVAGWIKRGPTGFIGTNKSCSMQTVQALV
ADFNDGRLTDPVATPTALDQLVQARQPQAIGCAGWRAIDAAEIARGSADGRVRNKFTDVA
EMLAAATSAPKEPLRRRVLARLRDLGQPIVLTVPL