Protein Info for Rv0861c in Mycobacterium tuberculosis H37Rv

Annotation: DNA helicase Ercc3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 PF13625: Helicase_C_3" amino acids 1 to 127 (127 residues), 131.5 bits, see alignment E=4.7e-42 PF04851: ResIII" amino acids 174 to 320 (147 residues), 64.9 bits, see alignment E=2.1e-21 PF00270: DEAD" amino acids 177 to 321 (145 residues), 26.6 bits, see alignment E=1.1e-09 PF16203: ERCC3_RAD25_C" amino acids 346 to 530 (185 residues), 138.3 bits, see alignment E=6.9e-44 PF00271: Helicase_C" amino acids 389 to 492 (104 residues), 48 bits, see alignment E=3.4e-16

Best Hits

KEGG orthology group: K10843, DNA excision repair protein ERCC-3 [EC: 3.6.4.12] (inferred from 100% identity to mtc:MT0884)

Predicted SEED Role

"DNA repair helicase"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (542 amino acids)

>Rv0861c DNA helicase Ercc3 (Mycobacterium tuberculosis H37Rv)
VQSDKTVLLEVDHELAGAARAAIAPFAELERAPEHVHTYRITPLALWNARAAGHDAEQVV
DALVSYSRYAVPQPLLVDIVDTMARYGRLQLVKNPAHGLTLVSLDRAVLEEVLRNKKIAP
MLGARIDDDTVVVHPSERGRVKQLLLKIGWPAEDLAGYVDGEAHPISLHQEGWQLRDYQR
LAADSFWAGGSGVVVLPCGAGKTLVGAAAMAKAGATTLILVTNIVAARQWKRELVARTSL
TENEIGEFSGERKEIRPVTISTYQMITRRTKGEYRHLELFDSRDWGLIIYDEVHLLPAPV
FRMTADLQSKRRLGLTATLIREDGREGDVFSLIGPKRYDAPWKDIEAQGWIAPAECVEVR
VTMTDSERMMYATAEPEERYRICSTVHTKIAVVKSILAKHPDEQTLVIGAYLDQLDELGA
ELGAPVIQGSTRTSEREALFDAFRRGEVATLVVSKVANFSIDLPEAAVAVQVSGTFGSRQ
EEAQRLGRILRPKADGGGAIFYSVVARDSLDAEYAAHRQRFLAEQGYGYIIRDADDLLGP
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