Protein Info for Rv0859 in Mycobacterium tuberculosis H37Rv

Annotation: Possible acyl-CoA thiolase FadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00108: Thiolase_N
  • amino acids 6 to 226 (221 residues), 155.3 bits, E=3.1e-49
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 7 to 401 (395 residues), 428.4 bits, E=1.3e-132
• PF00109: ketoacyl-synt
  • amino acids 92 to 123 (32 residues), 23.3 bits, see alignment (E = 7e-09)
• PF02803: Thiolase_C
  • amino acids 281 to 402 (122 residues), 160 bits, E=3.1e-51

Best Hits

Swiss-Prot: 100% identical to Y0859_MYCTU: Putative acyltransferase Rv0859 (fadA) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K00626, acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (inferred from 100% identity to mtf:TBFG_10876)

Predicted SEED Role

"3-ketoacyl-CoA thiolase [isoleucine degradation] (EC 2.3.1.16)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation (EC 2.3.1.16)

MetaCyc Pathways

• androstenedione degradation I (aerobic) (22/25 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (34/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (36/47 steps found)
• superpathway of testosterone and androsterone degradation (22/28 steps found)
• oleate β-oxidation (27/35 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• L-isoleucine degradation I (5/6 steps found)
• fatty acid salvage (5/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• ketolysis (2/3 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (12/17 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• acetoacetate degradation (to acetyl CoA) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• valproate β-oxidation (6/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• acetyl-CoA fermentation to butanoate (4/7 steps found)
• fatty acid β-oxidation I (generic) (4/7 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• polyhydroxybutanoate biosynthesis (1/3 steps found)
• 5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
• glycerol degradation to butanol (10/16 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (2/5 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• ketogenesis (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (14/22 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (1/4 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (5/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (17/27 steps found)
• ethylbenzene degradation (anaerobic) (1/5 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
• isopropanol biosynthesis (engineered) (1/5 steps found)
• pyruvate fermentation to acetone (1/5 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (2/7 steps found)
• mevalonate pathway II (haloarchaea) (2/7 steps found)
• L-lysine fermentation to acetate and butanoate (4/10 steps found)
• 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
• 4-ethylphenol degradation (anaerobic) (1/6 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (15/26 steps found)
• 2-deoxy-D-ribose degradation II (2/8 steps found)
• 2-methylpropene degradation (2/8 steps found)
• isoprene biosynthesis II (engineered) (2/8 steps found)
• mevalonate pathway III (Thermoplasma) (2/8 steps found)
• mevalonate pathway IV (archaea) (2/8 steps found)
• 3-phenylpropanoate degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• sitosterol degradation to androstenedione (8/18 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (2/10 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• 4-oxopentanoate degradation (1/9 steps found)
• ethylmalonyl-CoA pathway (2/11 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (3/13 steps found)
• androstenedione degradation II (anaerobic) (13/27 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (3/14 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (1/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (1/12 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• jasmonic acid biosynthesis (4/19 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• platensimycin biosynthesis (7/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• superpathway of cholesterol degradation III (oxidase) (23/49 steps found)
• superpathway of ergosterol biosynthesis I (6/26 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (23/56 steps found)
• superpathway of L-lysine degradation (10/43 steps found)
• superpathway of cholesterol biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Ethylbenzene degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Protein Sequence (403 amino acids)

>Rv0859 Possible acyl-CoA thiolase FadA (Mycobacterium tuberculosis H37Rv)
MSEEAFIYEAIRTPRGKQKNGSLHEVKPLSLVVGLIDELRKRHPDLDENLISDVILGCVS
PVGDQGGDIARAAVLASGMPVTSGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVE
SMSRVPMGSDGGAMGLDPATNYDVMFVPQSIGADLIATIEGFSREDVDAYALRSQQKAAE
AWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTTKEGLAKLKPAFEGLAALGGFDDVALQ
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSAAAGKLQGLTPRARIVATATSGADPVIML
TGPTPATRKVLDRAGLTVDDIDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHP
LGATGAMILGTMVDELERRNARRALITLCIGGGMGVATIIERV