Protein Info for Rv0753c in Mycobacterium tuberculosis H37Rv

Annotation: Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 6 to 494 (489 residues), 622.7 bits, E=2.1e-191
• PF00171: Aldedh
  • amino acids 17 to 490 (474 residues), 459.5 bits, E=1.1e-141
• PF05893: LuxC
  • amino acids 134 to 270 (137 residues), 27.7 bits, E=1.3e-10

Best Hits

Swiss-Prot: 45% identical to IOLA1_GEOKA: Malonate-semialdehyde dehydrogenase 1 (iolA1) from Geobacillus kaustophilus (strain HTA426)

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 100% identity to mbo:Mb0775c)

MetaCyc: 55% identical to malonate-semialdehyde dehydrogenase (acetylating) (Halomonas sp. HTNK1)
Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.18]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• L-valine degradation I (7/8 steps found)
• β-alanine degradation II (2/2 steps found)
• β-alanine degradation I (1/2 steps found)
• acrylate degradation I (3/5 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• 2,4-dinitrotoluene degradation (1/7 steps found)
• myo-inositol degradation I (1/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27

Protein Sequence (510 amino acids)

>Rv0753c Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH) (Mycobacterium tuberculosis H37Rv)
MTTQISHFIDGQRTAGQSTRSADVFDPNTGQIQAKVPMAGKSDIDAAVASAVEAQKGWAA
WNPQRRARVLMRFIELVNDTIDELAELLSREHGKTLADARGDVQRGIEVIEFCLGIPHLL
KGEYTEGAGPGIDVYSLRQPLGVVAGITPFNFPAMIPLWKAGPALACGNAFVLKPSERDP
SVPVRLAELFIEAGLPAGVFQVVHGDKEAVDAILHHPDIKAVGFVGSSDIAQYIYAGAAA
TGKRAQCFGGAKNHMIVMPDADLDQAVDALIGAGYGSAGERCMAISVAVPVGDQTAERLR
ARLIERINNLRVGHSLDPKADYGPLVTGAALARVRDYIGQGVAAGAELVVDGRDRASDDL
TFGLPEGDANLEGGFFIGPTLFDHVAAHMSIYTDEIFGPVLCMVRARDYEEALRLPSEHE
YGNGVAIFTRDGDAARDFVSRVQVGMVGVNVPIPVPVAYHTFGGWKRSGFGDLNQHGPAA
IQFYTKVKTVTSRWPSGIKDGAEFVIPTMS